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The first report on the detection and identification of phytoplasma pathogens associated with some ornamental plants of Yazd city
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Mehrdad Salehzadeh *   , Alireza Afsharifar , Saeedeh Dehghanpour farashah |
| Plant Virology Research Center, School of Agriculture, Shiraz University, Shiraz, I. R. Iran |
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Abstract: (751 Views) |
In spring and summer 2023, a survey of plant samples from green spaces and ornamental greenhouses, along with adjacent weeds in Yazd City, showed symptoms likely linked to phytoplasma disease. Notable symptoms included stem flattening, phyllody, and yellowing observed in Angelica archangelica, Ligustrum sp., Mirabilis jalapa, and Hibiscus cannabinus. Five samples from each plant were sent to the Plant Virology Research Center at Shiraz University for DNA extraction via the CTAB method. Polymerase chain reaction (PCR) was conducted using general primers (P1/P7) and nested primers (R16MF2/R16MR2) specific for phytoplasma. As expected, fragments of 1800 bp and 1250 bp were amplified with the general and nested primers, respectively. These fragments were excised from the gel, purified, and sent to Sinohe Company for sequencing. Comparison of the sequences with those in the NCBI database using BLAST showed that the phytoplasmas from Angelica archangelica, Ligustrum sp., Mirabilis jalapa, and Hibiscus cannabinus were closely related to Indian (MH393563.1; host: Datura stramonium; identity: 98.94%), Turkish (HE649495.1; host: Ligustrum sp.; identity: 99.24%), Ugandan (HE649495.1; host: Hibiscus rosae; identity: 99.14%), and Iranian (KX685880.1; host: Eucalyptus sp.; identity: 98.64%) isolates. A phylogenetic tree was created to compare Iranian isolates with similar global isolates using MEGA 8.0 software. |
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| Keywords: Phylogenetic analysis, Ornamental plants, Yellowing, Phyllody |
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Full-Text [PDF 678 kb]
(222 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2023/12/23 | Accepted: 2024/03/2 | Published: 2024/09/21
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