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Survey and identification of some plant pathogenic viruses from Dendranthema×grandiflorum, Ipomoea sp., and Dieffenbachia sp. from Iran
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Mehrdad Salehzadeh *   , Alireza Afsharifar , Saeedeh Dehghanpour farashah , Tayyebe Kyani-Bahram abadi |
| Plant Virology Research Center, School of Agriculture, Shiraz University, Shiraz, I. R. Iran |
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Abstract: (256 Views) |
The spread of plant pathogenic viruses is exacerbated by vegetative propagation, which facilitates rapid transmission. In the spring and summer of 2022, symptomatic samples of Dendranthema×grandiflorum plants exhibiting mosaic symptoms, leaf deformation, and occasional chlorotic spots were collected from green spaces in Tehran, Tabriz, Yazd, and Shiraz. Additionally, Ipomoea sp. samples with chlorotic spots were collected from the Faculty of Agriculture at Shiraz University, while Dieffenbachia sp. houseplant samples with mosaic symptoms and leaf deformities were obtained from greenhouses in Yazd. To identify the causative viral agents in these symptomatic plants, degenerate primers targeting significant virus families, as well as specific primers for the Cucumber mosaic virus, were utilized. Reverse transcription polymerase chain reaction (RT-PCR) with Tobamovirus-specific primers, based on a fragment of the viral coat protein, confirmed Tobacco mosaic virus (TMV) infection in symptomatic Dendranthema leaves, amplifying an 880 bp fragment. Additionally, RT-PCR on Ipomoea sp. and Dieffenbachia sp. samples with Potyvirus degenerate primers, targeting the nuclear inclusion body region, amplified 350 bp fragments. Subsequent sequencing confirmed Dasheen mosaic virus (DsMV) in Dieffenbachia sp. and Sweet potato virus G (SPVG) in Ipomoea sp. Control assays indicated no viral presence in asymptomatic plants. To our knowledge, this study presents the first report of Dendranthema, Ipomoea, and Dieffenbachia species infections with these viruses in Iran.
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| Keywords: Tobamovirus, Potyvirus, Tobacco mosaic virus, Dashin mosaic virus, Sweet potato virus G. |
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Full-Text [PDF 834 kb]
(83 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2024/03/17 | Accepted: 2024/07/17 | Published: 2024/11/22
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