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:: دوره 8، شماره 1 - ( بهار و تابستان 1402 ) ::
جلد 8 شماره 1 صفحات 200-183 برگشت به فهرست نسخه ها
ژن آنتی پورتر جلبک Zostera marina (ZmNa+/H+) به تنباکو زینتی (Nicotiana alata) تحمل به تنش شوری می دهد
سهیلا خورسندی ، پرستو احسانی ، ابوالفضل جوکار* ، عباس عالم زاده
دانشگاه شیراز
چکیده:   (452 مشاهده)
تنش شوری عامل اصلی محدود کننده رشد و نمو گیاهان است که در نتیجه تغییرهای آب و هوایی ایجاد می شود. تنباکو زینتی گیاهی از تیره سیب زمینی با گل های جذاب و مناسب مناطق خشک و نیمه خشک می باشد. برای ایجاد تحمل به شوری ژن آنتی پورتر Na+/H+   از گیاه مارماهی هالوفیت (Z. marina) رشد یافته در دریاچه آب شور ارومیه به گیاه تنباکو زینتی منتقل شد. برای انتقال ژن، ریز نمونه های برگ در محیط حاوی Agrobacteriumtumefaciens  حاوی ژن واکوئلی ZmNHX1 غوطه ور شدند. ریزنمونه ها روی محیط MS حاوی 1 میلی گرم در لیتر بنزیل آمینو پورین، 1/0 میلی گرم در لیتر نفتالن استیک اسید، 200 میلی گرم در لیتر سفوتاکسیم و 10 میلی گرم در لیتر کانامایسین کشت شدند. پس از باززایی، تنش شوری با غلظت‌های 0، 210، 230 و 240 میلی‌مولار کلرید سدیم در قالب طرح کاملاً تصادفی با سه تکرار اعمال شد.  ژن ZmNHX1 انتی پورتر Na+/H+  واکوئلی برای اولین بار با موفقیت به تنباکوهای زینتی منتقل شد. در مقایسه با گیاهان نوع وحشی، گیاهان تراریخته افزایش میزان کلروفیل، کاروتنوئید، وزن تر، وزن خشک، محتوای پرولین و فعالیت کاتالاز را نشان دادند. پس از تنش شوری، گیاهان تراریخته میزان کلروفیل، کاروتنوئید، آنتوسیانین، نسبت پتاسیم به سدیم، وزن تر، وزن خشک، مقدار پرولین، کاتالاز و آسکوربات پراکسیداز بیشتری را نشان دادند. گیاهان تراریخته همچنین کاهش کمتری در محتوای نسبی آب و افزایش کمتری در نشت الکترولیت نسبت به گیاهان نوع وحشی نشان دادند. نتایج نشان داد   ژن آنتی پورتر ZmNHX1 به دست آمده از علف مارماهی قابل استفاده بوده و تنباکوی زینتی تراریخته حاصل برای کشت در باغچه در مناطقی که در برابر تنش شوری قرار دارند پیشنهاد می شود.
واژه‌های کلیدی: Antiporter gene، Nicotina alata، Ornamental tobacco، Salt stress tolerance، ZmNHX1، Zostera marina.
متن کامل [PDF 614 kb]   (133 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: تخصصي
دریافت: 1401/10/25 | پذیرش: 1401/11/17 | انتشار: 1402/10/15
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Khorsandy S, Ehsani P, Jowkar A, Alemzadeh A. The eelgrass Zostera marina antiporter gene (ZmNa+/H+) confers salt stress tolerance to ornamental tobacco (Nicotiana alata). FOP 2023; 8 (1) :183-200
URL: http://flowerjournal.ir/article-1-268-fa.html

خورسندی سهیلا، احسانی پرستو، جوکار ابوالفضل، عالم زاده عباس. ژن آنتی پورتر جلبک Zostera marina (ZmNa+/H+) به تنباکو زینتی (Nicotiana alata) تحمل به تنش شوری می دهد. گل و گیاهان زینتی. 1402; 8 (1) :183-200

URL: http://flowerjournal.ir/article-1-268-fa.html



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