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:: Volume 4, Issue 1 (10-2019) ::
FOP 2019, 4(1): 47-61 Back to browse issues page
Comparison of the in vitro and in vivo culture of three cultivars of African violet (Saintpaulia ionantha H. Wendi.)
Mahmoud Fatahi Faradonbe , Maryam Dehestani Ardakani * , Kazem Kamali Aliabad
Assistant Professor, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, P.O. Box 184, Ardakan, Iran, Medicinal and Industrial Research Institute, Ardakan, Iran
Abstract:   (4360 Views)
African violet (Saintpaulia ionantha H. Wendi.) is an important ornamental plant due to its diverse colors and shapes. The present study was conducted to compare in vitro and in vivo propagation of this plant. In in vitro culture, effect of five types of explants (leaf blade, petiole, petiole without epiderm, whole inflorescence and flower without petals) in three cultivars (‘Cool Blue’, ‘Rondita’ and ‘Knight Rider’) on MS culture medium contained 0.1 mg/l NAA and 1 mg/l BA were investigated. In in vivo culture, leaf cuttings of three cultivars were cultured in wrapped plastic dishes contained 70% peat-moss and 30% perlite. Finally, length of propagation time and number of buds in both methods were investigated. Results showed that in each cultivar, type of suitable explants for propagation is different. In cv. ‘Cool Blue’ none of five explants did show significant difference in regeneration percentage, bud production and number of leaves. In cv. ‘Rondita’ leaf blade 91.66%, petiole 91.66% and petiole without epiderm 83.33% of explants and in cv. ‘Knight Rider’ leaf blade, whole inflorescence, flower without petals and petiole explants 100% of explants showed the highest regeneration percentage. By leaf cutting propagation method, cv. ‘Cool Blue’ showed the maximum (8.25) number of plantlets. Comparison of growing period length of in vitro and in vivo methods showed that in all the cultivars, period of culture to flowering time in in vitro culture method was longer (180 to 230 days) than in vivo culture (120 to 140 days). However, the number of produced buds in in vitro culture were significantly higher than in vivo culture.
Keywords: Proliferation, Explant, Leaf cutting, Flowering, Plantlet
Full-Text [PDF 1026 kb]   (1326 Downloads)    
Type of Study: Research | Subject: Special
Received: 2019/12/8 | Accepted: 2019/12/8 | Published: 2019/12/8
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Fatahi Faradonbe M, Dehestani Ardakani M, Kamali Aliabad K. Comparison of the in vitro and in vivo culture of three cultivars of African violet (Saintpaulia ionantha H. Wendi.). FOP 2019; 4 (1) :47-61
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Volume 4, Issue 1 (10-2019) Back to browse issues page
گل و گیاهان زینتی Flower and Ornamental Plants
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