Lisianthus (Eustoma grandiflorum (Raf.) Shinn.) is one of the most important cut flowers in the world. However, a relatively short vase life reduces its marketability. Usually, tragacanth gel and hot water treatment is applied on fruits and there are a few reports on cut flowers too. The aim of the present study was to evaluate the effects of tragacanth gel and hot water treatments on extending vase life and increasing the qualitative properties of lisianthus cut flowers. For this purpose, an experiment was conducted based on a completely randomized design with 13 treatments including three levels of tragacanth (1, 2.5 and 5%) in two ways (pulsing and continuous), hot water in 35 and 45 °C for 2, 5 and 10 min and control, all with three replications. The highest vase life, flower weight and the lowest bacteria population were observed in 2.5% tragacanth pulse treatment. Maximum catalase and peroxidase activity was obtained in 1% continuous tragacanth treatment. The highest relative water content of leaves and petals was observed in hot water in 35 °C for 2 min and 2.5 and 5% continuous tragacanth treatment, respectively. The highest ion leakage was observed in 2.5 and 5% continuous tragacanth treatment. The lowest ion leakage was obtained in hot water in 45 °C for 2 min. Generally, both tragacanth and hot water treatments were effective on extending vase life and improving quality properties of lisianthus, but tragacanth pulse treatment was the best.