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انجمن گل و گیاهان زینتی ایران
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انجمن گل و گیاهان زینتی ایران

پژوهشکده ملی گل و گیاهان زینتی
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:: دوره 9، شماره 1 - ( بهار و تابستان 1403 ) ::
جلد 9 شماره 1 صفحات 82-69 برگشت به فهرست نسخه ها
اولین گزارش از ردیابی و شناسایی بیمارگرهای فیتوپلاسمایی مرتبط با برخی از گیاهان زینتی شهر یزد
مهرداد صالح زاده* ، علیرضا افشاریفر ، سعیده دهقانپور فراشاه
مرکز تحقیقات ویروس شناسی گیاهی، دانشگاه شیراز، شیراز، ج. ا، ایران.
چکیده:   (1875 مشاهده)
در بازدیدهای بهار و تابستان سال 1402 از نمونه‌‌های گیاهی فضای سبز و گلخانه‌‌های زینتی و علف‌‌های هرز همجوار این گیاهان در شهر یزد، نشانه های مشکوک به بیماری فیتوپلاسمایی مانند کتابی‌‌شدن ساقه، ریزبرگی و زردی مرتبط با گیاهان سنبل‌‌ختایی، برگ‌‌نو، لاله عباسی و کنف‌‌وحشی دیده شد. از هر گیاه 5 نمونه جمع‌‌آوری و به مرکز تحقیقات ویروس‌‌شناسی گیاهی دانشگاه شیراز منتقل شد. استخراج دی.ان.ای. به روش CTAB انجام شد و واکنش زنجیره‌‌ای پلی‌‌مراز با آغازگرهای عمومی (P1 / P7) و آغازگرهای آشیانه‌‌ای (R16MF2 / R16MR2) فیتوپلاسماها صورت پذیرفت. برابر انتظار در پی.سی.آر. با آغازگرهای عمومی و آشیانه‌‌ای قطعاتی به‌‌ترتیب به اندازه‌‌ی 1800 و 1250 جفت‌‌باز تکثیر شد. قطعات حاصل از تکثیر پی.سی.آر. حاصل از آغازگرهای R16MF2 / R16MR2 از ژل برش یافته و بعد از خالص‌‌سازی جهت تعیین ترادف دوطرفه به شرکت سینوهه ارسال شدند. مقایسه توالی‌‌های به‌‌دست آمده با توالی‌‌های موجود درNCBI  توسط نرم‌‌افزارBLAST  نشان داد که فیتوپلاسمای همراه با  گیاهان سنبل ختایی، برگ نو، لاله عباسی و کنف حشی بیشترین شباهت را به ترتیب با جدایه‌‌ هندی (MH393563.1; host; Datura stramonium; identity: 98.94%)، جدایه‌‌ ترکیه‌‌ای (HE649495.1; host; Ligustrum sp.; identity: 99.24% جدایه‌‌ اوگاندایی (HE649495.1; host; Hibicus rosae;. identity: 99.14%) و جدایه‌‌ ایرانی (KX685880.1; host; Eucalyptus sp. identity: 98.64%) داشتند. در ادامه درخت تبارزایی برای مقایسه‌‌ جدایه‌‌های ایرانی با سایر جدایه‌‌های مشابه گزارش شده جهان با نرم‌‌افزار MEGA 8.0  ترسیم گردید.
واژه‌های کلیدی: آنالیز تبارزائی، گیاهان زینتی، زردی، علف‌‌های هرز، فیلودی
متن کامل [PDF 678 kb]   (584 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: تخصصي
دریافت: 1402/10/2 | پذیرش: 1402/12/12 | انتشار: 1403/6/31
فهرست منابع
1. Ashnaei, M., Jamshidi, E. (2016, August). First report of association of a 16SrVI phytoplasma with pot marigold phyllody in Khorasan Razavi province. In Iranian Plant Protection Congress (pp. 27-30).
2. Amirmijani, A. R., Salari, M. R., Amirmijani, A., Khoshsohbat, M. S., Azadvar, M. (2020). First Report of Association of a 'Candidatus Phytoplasma aurantifolia'16SrII-D Related Phytoplasma with Geranium (Pelargonium hortorum) Little Leaf Disease in Iran. Plant Disease, 104(10), 2723. [DOI:10.1094/PDIS-03-20-0561-PDN]
3. Aldaghi, M., Bertaccini, A. (2015). Preliminary study on some ornamental plant phytoplasma diseases in north of Iran. Phytopathogenic Mollicutes, 5(1s), S67-S68. http://dx.doi.org/10.5958/2249-4677.2015.00028.6. [DOI:10.5958/2249-4677.2015.00028.6]
4. Azimi, M., Mehrabi‐Koushki, M., Farokhinejad, R. (2018). Association of two groups of phytoplasma with various symptoms in some wooden and herbaceous plants. Journal of Phytopathology, 166(4), 273-282. https://doi.org/10.1111/jph.12684 [DOI:10.1111/jph.12684.]
5. Amirmijani, A. R., Salari, M. R., Amirmijani, A., Khoshsohbat, M. S., Azadvar, M. (2020). First Report of Association of a 'Candidatus Phytoplasma aurantifolia'16SrII-D Related Phytoplasma with Geranium (Pelargonium hortorum) Little Leaf Disease in Iran. Plant Disease, 104(10), 2723. https://doi.org/10.1094/PDIS-03-20-0561-PDN [DOI:10.1094/PDIS-03-20-0561-PDN.]
6. Babaie, G., Khatabi, B., Bayat, H., Rastgou, M., Hosseini, A., Salekdeh, G. H. (2007). Detection and characterization of phytoplasmas infecting ornamental and weed plants in Iran. Journal of Phytopathology, 155(6), 368-372. https://doi.org/10.1111/j.1439-0434.2007.01247.x [DOI:10.1111/j.1439-0434.2007.01247.x.]
7. Bertaccini, A., Arocha-Rosete, Y., Contaldo, N., Duduk, B., Fiore, N., Montano, H. G., Zamorano, A. (2022). Revision of the 'Candidatus Phytoplasma'species description guidelines. International Journal of Systematic and Evolutionary Microbiology, 72(4), 005353. https://doi.org/10.1099/ijsem.0.005353 [DOI:10.1099/ijsem.0.005353.]
8. Biswas, C., Dey, P., Satpathy, S. (2014). First report on molecular detection of a stolbur phytoplasma (Group16SrXII-A) associated with kenaf (Hibiscus cannabinus L.) in India. Archives of Phytopathology and Plant Protection, 47(14), 1746-1751. [DOI:10.1080/03235408.2013.856549]
9. Çağlar, B. K., Pehlivan, S., Atakan, E., Elbeaino, T. (2021). Maize (Zea mays L.): A New Host for Ligustrum witches' Broom Phytoplasma. Pathogens, 10(6), 723. [DOI:10.3390/pathogens10060723]
10. Chaturvedi, Y., Rao, G., Tiwari, A., Duduk, B., Bertaccini, A. (2010). Phytoplasma on ornamentals: detection, diversity and management. Acta Phytopathologica et Entomologica Hungarica, 45(1), 31-69. https://doi.org/10.1556/APhyt.45.2010.1.3 [DOI:10.1556/aphyt.45.2010.1.3.]
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13. Gibbs, A., Mackenzie, A. (1997). A primer pair for amplifying part of the genome of all potyvirids by RT-PCR. Journal of Virological Methods, 63(1-2), 9-16. doi:10.1016/S0166-0934(96)02103-9. https://doi.org/10.1016/S0166-0934(96)02103-9 [DOI:10.1111/jph.12838.]
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15. Hemmati, C., Nikooei, M., Bertaccini, A. (2019). Identification, occurrence, incidence and transmission of phytoplasma associated with Petunia violacea witches' broom in Iran. Journal of Phytopathology, 167(10), 547-552. [DOI:10.1111/jph.12838]
16. Hugenholtz, P., Chuvochina, M., Oren, A., Parks, D. H., Soo, R. M. (2021). Prokaryotic taxonomy and nomenclature in the age of big sequence data. the ISME Journal, 15(7), 1879-1892. https://doi.org/10.1038/s41396-021-00941-x [DOI:10.1038/s41396-021-00941-x.]
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19. Mahapatra, B. S. Chinmay Biswas, Piyali Dey, Subrata Satpathy, Manoj Kumar, Pratik Satya. Bertaccini, A. (2018). Bertaccini, Phytoplasma diseases of medicinal and aromatic plants. Journal of Plant Pathology, 379-404. https://www.jstor.org/stable/44280481
20. Marcone, C., Franco-Lara, L., Toševski, I. (2018). Major phytoplasma diseases of forest and urban trees. Phytoplasmas: Plant pathogenic bacteria-I: Characterisation and epidemiology of phytoplasma-associated diseases, 287-312. https://doi.org/10.1007/978-981-13-0119-3_10 [DOI:10.1007/978-981-13-0119-3_10.]
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23. Mokbel, S. A., El-Attar, A. K. (2014). Effect of gamma irradiation on production of phytoplasma-free Hibiscus plantlets. Egyptian J. Virol, 11(2), 35-45. [DOI:10.1111/aab.12810]
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41. Aldaghi, M., Bertaccini, A. (2015). Preliminary study on some ornamental plant phytoplasma diseases in north of Iran. Phytopathogenic Mollicutes, 5(1s), S67-S68. http://dx.doi.org/10.5958/2249-4677.2015.00028.6. [DOI:10.5958/2249-4677.2015.00028.6]
42. Azimi, M., Mehrabi‐Koushki, M., Farokhinejad, R. (2018). Association of two groups of phytoplasma with various symptoms in some wooden and herbaceous plants. Journal of Phytopathology, 166(4), 273-282. https://doi.org/10.1111/jph.12684 [DOI:10.1111/jph.12684.]
43. Amirmijani, A. R., Salari, M. R., Amirmijani, A., Khoshsohbat, M. S., Azadvar, M. (2020). First Report of Association of a 'Candidatus Phytoplasma aurantifolia'16SrII-D Related Phytoplasma with Geranium (Pelargonium hortorum) Little Leaf Disease in Iran. Plant Disease, 104(10), 2723. https://doi.org/10.1094/PDIS-03-20-0561-PDN [DOI:10.1094/PDIS-03-20-0561-PDN.]
44. Babaie, G., Khatabi, B., Bayat, H., Rastgou, M., Hosseini, A., Salekdeh, G. H. (2007). Detection and characterization of phytoplasmas infecting ornamental and weed plants in Iran. Journal of Phytopathology, 155(6), 368-372. https://doi.org/10.1111/j.1439-0434.2007.01247.x [DOI:10.1111/j.1439-0434.2007.01247.x.]
45. Bertaccini, A., Arocha-Rosete, Y., Contaldo, N., Duduk, B., Fiore, N., Montano, H. G., Zamorano, A. (2022). Revision of the 'Candidatus Phytoplasma'species description guidelines. International Journal of Systematic and Evolutionary Microbiology, 72(4), 005353. https://doi.org/10.1099/ijsem.0.005353 [DOI:10.1099/ijsem.0.005353.]
46. Biswas, C., Dey, P., Satpathy, S. (2014). First report on molecular detection of a stolbur phytoplasma (Group16SrXII-A) associated with kenaf (Hibiscus cannabinus L.) in India. Archives of Phytopathology and Plant Protection, 47(14), 1746-1751. [DOI:10.1080/03235408.2013.856549]
47. Çağlar, B. K., Pehlivan, S., Atakan, E., Elbeaino, T. (2021). Maize (Zea mays L.): A New Host for Ligustrum witches' Broom Phytoplasma. Pathogens, 10(6), 723. [DOI:10.3390/pathogens10060723]
48. Chaturvedi, Y., Rao, G., Tiwari, A., Duduk, B., Bertaccini, A. (2010). Phytoplasma on ornamentals: detection, diversity and management. Acta Phytopathologica et Entomologica Hungarica, 45(1), 31-69. https://doi.org/10.1556/APhyt.45.2010.1.3 [DOI:10.1556/aphyt.45.2010.1.3.]
49. Duduk, B., Stepanović, J., Yadav, A., Rao, G. P. (2018). Phytoplasmas in weeds and wild plants. Phytoplasmas: Plant Pathogenic Bacteria-I: Characterisation and Epidemiology of Phytoplasma-Associated Diseases, 313-345. https://doi.org/10.1007/978-981-13-0119-3_11 [DOI:10.1007/978-981-13-0119-3_11.]
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51. Gibbs, A., Mackenzie, A. (1997). A primer pair for amplifying part of the genome of all potyvirids by RT-PCR. Journal of Virological Methods, 63(1-2), 9-16. doi:10.1016/S0166-0934(96)02103-9. https://doi.org/10.1016/S0166-0934(96)02103-9 [DOI:10.1111/jph.12838.]
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53. Hemmati, C., Nikooei, M., Bertaccini, A. (2019). Identification, occurrence, incidence and transmission of phytoplasma associated with Petunia violacea witches' broom in Iran. Journal of Phytopathology, 167(10), 547-552. [DOI:10.1111/jph.12838]
54. Hugenholtz, P., Chuvochina, M., Oren, A., Parks, D. H., Soo, R. M. (2021). Prokaryotic taxonomy and nomenclature in the age of big sequence data. the ISME Journal, 15(7), 1879-1892. https://doi.org/10.1038/s41396-021-00941-x [DOI:10.1038/s41396-021-00941-x.]
55. Jung, H. Y. (2023). Phytoplasma diseases in Korea. In Diversity, Distribution, and Current Status (pp. 233-248). Academic Press. https://doi.org/10.1016/B978-0-323-91896-1.00010-6 [DOI:10.1016/B978-0-323-91896-1.00010-6.]
56. Kumar, S., Singh, V., Lakhanpaul, S. (2012). First report of Mirabilis and Chrysanthemum little leaf associated with 'Candidatus Phytoplasma aurantifolia' in India. Australasian Plant Disease Notes, 7, 71-73. [DOI:10.1007/s13314-012-0051-0]
57. Mahapatra, B. S. Chinmay Biswas, Piyali Dey, Subrata Satpathy, Manoj Kumar, Pratik Satya. Bertaccini, A. (2018). Bertaccini, Phytoplasma diseases of medicinal and aromatic plants. Journal of Plant Pathology, 379-404. https://www.jstor.org/stable/44280481
58. Marcone, C., Franco-Lara, L., Toševski, I. (2018). Major phytoplasma diseases of forest and urban trees. Phytoplasmas: Plant pathogenic bacteria-I: Characterisation and epidemiology of phytoplasma-associated diseases, 287-312. https://doi.org/10.1007/978-981-13-0119-3_10 [DOI:10.1007/978-981-13-0119-3_10.]
59. Marcone, C., Bellardi, M. G., Bertaccini, A. (2016). Phytoplasma diseases of medicinal and aromatic plants. Journal of Plant Pathology, 379-404.
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Salehzadeh M, Afsharifar A, Dehghanpour farashah S. The first report on the detection and identification of phytoplasma pathogens associated with some ornamental plants of Yazd city. FOP 2024; 9 (1) :69-82
URL: http://flowerjournal.ir/article-1-296-fa.html

صالح زاده مهرداد، افشاریفر علیرضا، دهقانپور فراشاه سعیده. اولین گزارش از ردیابی و شناسایی بیمارگرهای فیتوپلاسمایی مرتبط با برخی از گیاهان زینتی شهر یزد. گل و گیاهان زینتی. 1403; 9 (1) :69-82

URL: http://flowerjournal.ir/article-1-296-fa.html



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گل و گیاهان زینتی Flower and Ornamental Plants
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