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Effects of different levels of benzyl adenine on in vitro regeneration and growth of Venus flytrap (Dionaea muscipula Ellis.)
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Manijhe Eslami   , Mahnaz Karimi * , Hossein Moradi |
| Sari Agricultural Sciences and Natural Resources University |
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Abstract: (434 Views) |
The Venus flytraps (Dionaea muscipula Ellis.) is an endangered ornamental plant, which little study has been done on its micropropagation. Cytokinins are a group of plant growth hormones that stimulate cell division, cell differentiation, stem initiation and reproductive growth in many plants. In this group, there are different types of cytokinins that can affect the physiology, growth and development of plants. Benzyl adenine is the adenine type of this group. The aim of this study was to investigate the effect of different concentrations of benzyl adenine on the regeneration and growth of Venus flytrap.
For this purpose, an experiment was conducted as a completely randomized design with 3 replications. Seedlings were grown in Murashig and Skoog culture medium with different concentrations of benzyl adenine (0.1, 0.3 and 0.5 mg/l). According to the obtained results, the effect of different concentrations of benzyl adenine on the rooting index and the number of roots was significant at the level of one percent probability and on the regeneration index, the number of leaves and the number of embryos at the level of five percent probability. The highest rooting index with 99.97% was in the concentration of 0.1 mg/l of benzyl adenine and the lowest was related to the concentration of 0.3 mg/l. The highest chlorophyll b, total chlorophyll and carotenoids were recorded in the concentration of 0.1 mg/liter of benzyl adenine. The highest ratio of chlorophyll a/b was obtained at a concentration of 0.5 mg/l of benzyl adenine. According to the results of the present study, the concentration of 0.1 mg/l of benzyl adenine has a significant effect compared to two concentrations of 0.3 and 0.5 mg/l on regeneration index, rooting index, number of roots and chlorophyll and carotenoid content.
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| Keywords: Cytokinin, Tissue Culture, Chlorophyll, Ornamental plant |
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Full-Text [PDF 600 kb]
(87 Downloads)
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Type of Study: Applicable |
Subject:
Special
Received: 2024/05/19 | Accepted: 2024/10/18 | Published: 2025/02/26
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