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Volume 8, Issue 2 (Fall and Winter 2023) |
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Investigating the hybridization of Lilium ledebourii Boiss with some commercial lilium cultivars and the possibility of in ovulo culture in their crosses
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Younes Mahdavi Fikejvar   , Pejman Azadi * , Freshteh Nematolahi |
| Agricultural Research, Education and Extension Organization (AREEO) |
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Abstract: (836 Views) |
Susan-e-Chelcharagh (Lilium ledebourii) is a rare native plant in Iran, primarily found in the elevated terrains of the Alborz region. This study explores the potential for crossbreeding L. ledebourii with commercially available lily varieties. Initially, the study compares the cytogenetic profile of L. ledebourii with several commercial cultivars. Subsequently, the feasibility of hybridization between L. ledebourii and a subset of commercial lily cultivars were analyzed. The study also examines the viability of in ovulo culture in maternal genotypes of Brindisi and Paradero cultivars when cross-pollinated with L. ledebourii pollen. Moreover, the effects of different time intervals (10, 20, and 30 days after-pollination) and NAA concentrations (ranging from 0 mg/L to 2.0 mg/L) on ovule development within MS medium were considered. The findings reveal that L. ledebourii is a diploid plant with functional pistils and pollen grains. However, when used as the maternal parent in crossings with commercial cultivars, all pistils undergo abscission, preventing fruit set. Additionally, fruit set is conspicuously absent when L. ledebourii serves as the paternal parent in crosses with the Companion, Fangio, and Arvandrud cultivars, and fruit formation is transient in other examined cultivars, disappearing completely by the eighth week post-pollination. The results of the in ovulo culture assays highlight the significant influence of isolation time and NAA concentration on ovule viability and enlargement. Notably, in the Brindisi cultivar, ovule germination becomes apparent when isolated 30 days after-pollination and supplemented with 0.1 mg/L NAA. In conclusion, interspecies hybridization involving L. ledebourii as the pollinating parent and select commercial cultivars remains possible by skillfully managing pre- and post-fertilization barriers through using optimal cultural media. |
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| Keywords: Flow cytometry, In ovulo culture, Ploidy level, Susan-e- Chelcheragh |
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Full-Text [PDF 704 kb]
(159 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2022/11/12 | Accepted: 2023/01/14 | Published: 2024/04/2
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Mahdavi Fikejvar Y, Azadi P, Nematolahi F. Investigating the hybridization of Lilium ledebourii Boiss with some commercial lilium cultivars and the possibility of in ovulo culture in their crosses. FOP 2023; 8 (2) :327-340 URL: http://flowerjournal.ir/article-1-259-en.html
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