Appraisal of leaf cutting, soil mixture and leaf explants on production of Sansevieria trifasciata under ex/in vitro condition

Dolati, Matin; Abasabad, Maryam; Seyfi, Esmaeil; Alizadeh, Mahdi; khoshhal.sarmast, Mostafa

doi:10.61186/flowerjournal.7.2.261

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Volume 7, Issue 2 (Fall and Winter 2023)

FOP 2023, 7(2): 261-276

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Appraisal of leaf cutting, soil mixture and leaf explants on production of Sansevieria trifasciata under ex/in vitro condition

, Mostafa Khoshhal.sarmast ^*

Gorgan University

Abstract: (1413 Views)

Mass propagation along with preserving the true-to-type characteristics is the goal of ornamental plants clonal propagation. Among the ornamental species, chimeric plants have always been of interest because of their attractive appearance. The mother-in-law's tongue or Sansevieria trifasciata, due to its fleshy leaves and high tolerance to adverse environmental conditions, is very suitable as an indoor plant. Since the clonal propagation of aforesaid plants are through rhizomes and offset, therefore the aim of this study was to evaluate the type of leaf cuttings and soil mixture under greenhouse conditions and also aimed to see leaf explants response under in vitro condition. The results of experiments performed in the greenhouse show that the type of leaf cuttings is ineffective in inducing chimeric plants. Cocopeat-Perlite soil mixture led to the production of more roots in regenerated plants, however, compared to Perlite, it failed to improve shoot regeneration. There was no significant difference between plants regenerated from chimeric parts of reverse V shape cuttings compared to the simple leaf cuttings. In vitro studies using the 2,4-D and kinetin revealed that the explants which were incubated for 20 days in MS media supplemented with 0.35 mg/L 2,4-D and then kinetin resulted in a higher average number of shoots. Although the use of leaf explant was not effective in inducing true chimeric shoots, some plastidial mutations were seen, leading to the production of albino plants. In vitro-induced plants were acclimated with 100% efficiency in greenhouse conditions under mist system. Overall, the results of these in/ex vitro studies failed to induce chimeric plants likely due to the de novo meristemoid induction originating from inner layers of chimeric tissues.

Keywords: Chimeric tissue, Leaf cutting, Proliferation, Sansevieria trifasciata, 2, 4-D

Full-Text [PDF 933 kb] (321 Downloads)

Type of Study: Research | Subject: Special
Received: 2022/06/27 | Accepted: 2022/08/26 | Published: 2023/06/18

References

1. Blazich, F.A., Novitzky, R.T. (1984). In vitro propagation of Sansevieria trifasciata. HortScience, 19(1), 122-123. [DOI:10.21273/HORTSCI.19.1.122]

2. Donnelly, D.J., Ortiz-Medina, E. (2005). Micropropagation and genetic risk: securing clonal fidelity. Proceedings of The International Workshop On True-To-Typeness of Date Palm Tissue Culure-Derived Plants Morocco.

3. Frank, M.H., Chitwood, D.H. (2016). Plant chimeras: Thegood,thebad,andthe 'Bizzaria'. Developmental Biology, 419, 41-53. [DOI:10.1016/j.ydbio.2016.07.003]

4. Frello, S. Venerus E., Serek, M. (2002). Regeneration of various species of Crassulaceae, with special reference to Kalanchoe. Journal of Horticultural Science and Biotechnology, 77, 204-208. [DOI:10.1080/14620316.2002.11511480]

5. García Hernández, E., Loera Quezada, M.M., Morán Velázquez, D.C., López, M.G., Chable Vega, M.A, Santillán Fernández, A., Zavaleta Mancera, H.A., Tang, J.Z., Azadi, P., Ibarra Laclette, E., Alatorre Cobos, F. (2022). Indirect organogenesis for high frequency shoot regeneration of two cultivars of Sansevieria trifasciata Prain differing in fiber production. Scientific Report, 12, 8507. [DOI:10.1038/s41598-022-12640-4]

6. Henley, R.W., Chase, A.R., Osborne. L.S. Sansevieria Production Guide.CFREC-A Foliage Plant Note RH-91-30. University of Florida, USA.

7. Ioannou, M., Ioannou, N. (1992). Micropropagation of Kalanchoe Blossfeldiana Poelln., from leaf-blade segments. Miscellaneous Reports Agricultural Research Institute, Ministry of Agriculture and Natural Resources Nicosia, 53, 4.

8. Kakoui, F., Salehi, H. (2011). The effect of different pot mixes on growth and development of Spathiphyllum wallisii. 7th Congress of Iranian Horticultural Science, Esfahan, Iran.

9. Kaur, J., Mudgal, G. (2021). An efficient and quick protocol for in vitro multiplication of snake plant, Sansevieria trifasciata var. Laurentii. Plant Cell Tissue and Organ Culture, 147, 405-411. https://doi.org/10.1007/s11240-021-02132-0 [DOI:10.1007/s11240-021-02132-0.]

10. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologiae Plantarum, 15, 473-497. [DOI:10.1111/j.1399-3054.1962.tb08052.x]

11. Nazari, F., Farahmand, H., Ghasemi Ghehsareh, M. (2014). The effects of different amounts of natural zeolite on vegetative and reproductive characteristics of Narcissus tazetta L. cv. Shahla. Journal of Plant Production, 37, 39-48.

12. Paek, K.Y., Hahn, E.J. (1999). Variations in African violet 'Crimson Frost' micropropagated by homogenized leaf tissue culture. HortTechnology, 9. https://doi.org/10.21273/HORTTECH.9.4.625 [DOI:10.21273/HORTTECH.9.4.625.]

13. Samiei, L., Khalighi A, Kafi M and Samavat S. (2005). Peat moss substituting with some organic wastes in pothos (Epipremnum aureum 'Golden pothos') growing media. Journal of Horticulture Technology and Science, 6(2), 79 - 88.

14. Sarmast, M.K., Salehi, M., Salehi, H. (2009). The potential of different parts of Sansevieria trifasciata L. leaf for meristemoids production. Australian Journal of Basic and Applied Sciences, 3(3), 2506-2509.

15. Shahzadm, A., Ahmasd, N., Rather, M.A., Husain, M.K., Anis, M. (2009). Improved shoot regeneration system through leaf derived callus and nodule culture of Sansevieria cylindrical. Biologia Plantarum, 53, 745-749. [DOI:10.1007/s10535-009-0136-6]

16. Torres, K.C. (1989). In Vitro Propagation of Snake Plant (Sansevieria). In: Tissue Culture Techniques for Horticultural Crops. Van Nostrand Reinhold. [DOI:10.1007/978-1-4615-9756-8_8]

17. Veltcheva, M.R., Svetleva, D.L. (2005). In vitro regeneration of Phaseolus vulgaris L. via organogenesis from petiole ex-plants. Journal of Central European Agriculture, 6(1), 53-58.

18. Wahyuningsih, T. (2006). Pengaruh beberapa konsentrasi benziladenin (BA) atau kinetin pada pembentukan tunas adventif Sansevieria trifasciata Lorentii in vitro. Skripsi Fakultas Pertanian, Universitas Lampung. pp.26.

19. Wang, Y., Mou, H., Lü, Y., Li, H., Wang, Y., Chen, J. (2017). In vitro Regeneration and Industrial Micropropagation of Haworthia retusa × cooperi cv. 'Variegata'. Chinese Bulletin of Botany, 52, 331-336. [DOI:10.21273/HORTSCI11413-16]

20. Yusnita Pungkastiani, W., Hapsoro, D. (2011). In vitro organogenesis of two Sansevieria cultivars on different concentration of benzyladenine (BA). Agrivita, 147-153.

21. Blazich, F.A., Novitzky, R.T. (1984). In vitro propagation of Sansevieria trifasciata. HortScience, 19(1), 122-123. [DOI:10.21273/HORTSCI.19.1.122]

22. Donnelly, D.J., Ortiz-Medina, E. (2005). Micropropagation and genetic risk: securing clonal fidelity. Proceedings of The International Workshop On True-To-Typeness of Date Palm Tissue Culure-Derived Plants Morocco.

23. Frank, M.H., Chitwood, D.H. (2016). Plant chimeras: Thegood,thebad,andthe 'Bizzaria'. Developmental Biology, 419, 41-53. [DOI:10.1016/j.ydbio.2016.07.003]

24. Frello, S. Venerus E., Serek, M. (2002). Regeneration of various species of Crassulaceae, with special reference to Kalanchoe. Journal of Horticultural Science and Biotechnology, 77, 204-208. [DOI:10.1080/14620316.2002.11511480]

25. García Hernández, E., Loera Quezada, M.M., Morán Velázquez, D.C., López, M.G., Chable Vega, M.A, Santillán Fernández, A., Zavaleta Mancera, H.A., Tang, J.Z., Azadi, P., Ibarra Laclette, E., Alatorre Cobos, F. (2022). Indirect organogenesis for high frequency shoot regeneration of two cultivars of Sansevieria trifasciata Prain differing in fiber production. Scientific Report, 12, 8507. [DOI:10.1038/s41598-022-12640-4]

26. Henley, R.W., Chase, A.R., Osborne. L.S. Sansevieria Production Guide.CFREC-A Foliage Plant Note RH-91-30. University of Florida, USA.

27. Ioannou, M., Ioannou, N. (1992). Micropropagation of Kalanchoe Blossfeldiana Poelln., from leaf-blade segments. Miscellaneous Reports Agricultural Research Institute, Ministry of Agriculture and Natural Resources Nicosia, 53, 4.

28. Kakoui, F., Salehi, H. (2011). The effect of different pot mixes on growth and development of Spathiphyllum wallisii. 7th Congress of Iranian Horticultural Science, Esfahan, Iran.

29. Kaur, J., Mudgal, G. (2021). An efficient and quick protocol for in vitro multiplication of snake plant, Sansevieria trifasciata var. Laurentii. Plant Cell Tissue and Organ Culture, 147, 405-411. https://doi.org/10.1007/s11240-021-02132-0 [DOI:10.1007/s11240-021-02132-0.]

30. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologiae Plantarum, 15, 473-497. [DOI:10.1111/j.1399-3054.1962.tb08052.x]

31. Nazari, F., Farahmand, H., Ghasemi Ghehsareh, M. (2014). The effects of different amounts of natural zeolite on vegetative and reproductive characteristics of Narcissus tazetta L. cv. Shahla. Journal of Plant Production, 37, 39-48.

32. Paek, K.Y., Hahn, E.J. (1999). Variations in African violet 'Crimson Frost' micropropagated by homogenized leaf tissue culture. HortTechnology, 9. https://doi.org/10.21273/HORTTECH.9.4.625 [DOI:10.21273/HORTTECH.9.4.625.]

33. Samiei, L., Khalighi A, Kafi M and Samavat S. (2005). Peat moss substituting with some organic wastes in pothos (Epipremnum aureum 'Golden pothos') growing media. Journal of Horticulture Technology and Science, 6(2), 79 - 88.

34. Sarmast, M.K., Salehi, M., Salehi, H. (2009). The potential of different parts of Sansevieria trifasciata L. leaf for meristemoids production. Australian Journal of Basic and Applied Sciences, 3(3), 2506-2509.

35. Shahzadm, A., Ahmasd, N., Rather, M.A., Husain, M.K., Anis, M. (2009). Improved shoot regeneration system through leaf derived callus and nodule culture of Sansevieria cylindrical. Biologia Plantarum, 53, 745-749. [DOI:10.1007/s10535-009-0136-6]

36. Torres, K.C. (1989). In Vitro Propagation of Snake Plant (Sansevieria). In: Tissue Culture Techniques for Horticultural Crops. Van Nostrand Reinhold. [DOI:10.1007/978-1-4615-9756-8_8]

37. Veltcheva, M.R., Svetleva, D.L. (2005). In vitro regeneration of Phaseolus vulgaris L. via organogenesis from petiole ex-plants. Journal of Central European Agriculture, 6(1), 53-58.

38. Wahyuningsih, T. (2006). Pengaruh beberapa konsentrasi benziladenin (BA) atau kinetin pada pembentukan tunas adventif Sansevieria trifasciata Lorentii in vitro. Skripsi Fakultas Pertanian, Universitas Lampung. pp.26.

39. Wang, Y., Mou, H., Lü, Y., Li, H., Wang, Y., Chen, J. (2017). In vitro Regeneration and Industrial Micropropagation of Haworthia retusa × cooperi cv. 'Variegata'. Chinese Bulletin of Botany, 52, 331-336. [DOI:10.21273/HORTSCI11413-16]

40. Yusnita Pungkastiani, W., Hapsoro, D. (2011). In vitro organogenesis of two Sansevieria cultivars on different concentration of benzyladenine (BA). Agrivita, 147-153.

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Dolati M, Abasabad M, Seyfi E, Alizadeh M, khoshhal.sarmast M. Appraisal of leaf cutting, soil mixture and leaf explants on production of Sansevieria trifasciata under ex/in vitro condition. FOP 2023; 7 (2) :261-276
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