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:: Volume 6, Issue 1 (Spring and Summer 2021) ::
FOP 2021, 6(1): 1-12 Back to browse issues page
ISSR-based genetic diversity assessment of five populations of Juniperus polycarpos K. Koch in southern habitats of Iran
Abdolrahman Rahimian Boogar , Hassan Salehi
Department of Horticultural Science and landscape Engineering, Faculty of Agriculture, University of Zabol, Zabol, Iran (Present address).
Abstract:   (353 Views)
Juniperus polycarpos K. Koch is a durable conifer species with different growth habit that grow in ecological landscapes. In this study, genetic diversity was investigated for five populations of juniper that grew in Fasa, Sepidan, Khabr, Rabor, and Genow habitat in the south of Iran. For DNA extraction, leaf samples of 10 diverse accessions of each habitat were collected (totally 50 accessions). Populations genetic diversity was evaluated based on 12 inter simple sequence repeat (ISSR) markers. Assessment of ISSR markers predicted 75 loci with 67 (89.33%) polymorphic loci, polymorphic information content (PIC) 0.45, resolving power (Rp) 3.78, and effective multiplex ratio (EMR) and marker index (MI) of primers were 5.50 and 2.47, respectively. Populations Nei’s genetic diversity showed higher intra-population genetic diversity (0.25) than inter-population genetic diversity (0.13). Results indicated the highest different loci (Na) for Sepidan population (56) and the highest effective alleles (Ne) were obtained in two populations of Sepidan (1.49) and Genow (1.49). Furthermore, the highest gene diversity (H) observed in the population of Sepidan (0.27) and Genow (0.27). The population of Sepidan has the highest Shannon’s information index (I) (0.41) and percentage of polymorphic loci (P) (74.67). Investigated populations showed high total genetic diversity (Ht) (0.38), intra-population genetic diversity (Hs) (0.25), and inter-population genetic diversity (Dst) (0.13), and moderate genetic differentiation among populations (Gst = 0.34). High gene flow (Nm) was obtained between evaluated populations (0.94). Moreover, AMOVA analysis indicated 70% within and 30% among populations genetic variation.
Keywords: Accession, Conifer, Juniper, Molecular markers, Variation
Full-Text [PDF 445 kb]   (178 Downloads)    
Type of Study: Research | Subject: Special
Received: 2021/08/14 | Accepted: 2021/09/22 | Published: 2022/01/15
References
1. Adams, R.P. (2004). Junipers of the World: the genus Juniperus. Vancouver, BC ‌- Trafford.
2. Adams, R.P. (2008). Junipers of the World: the genus Juniperus (2nd Ed), Vancouver, BC - Trafford.
3. Adams, R.P. (2014). Junipers of the world: The genus Juniperus (3rd Ed). Victoria, BC - Trafford.
4. Adams, R.P., Al-Farsi, A., Schwarzback, A. (2014). Confirmation of the southernmost population of Juniperus seravschanica in Oman by DNA sequencing of nrDNA and four cpDNA regions. Phytologia, 96 (3), 218-224.
5. Al-Turki. T.A., Basahi, M.A. (2015). Assessment of ISSR based molecular genetic diversity of Hassawi rice in Saudi Arabia. Saudi Journal of Biological Sciences, 22, 591-599. [DOI:10.1016/j.sjbs.2015.06.027]
6. Anderson, J.A., Churchill, G.A., Autrique, J.E., Tanksley, S.D., Sorrells, M.E. (1993). Optimizing parental selection for genetic linkage maps. Genome, 36(1), 181-186. [DOI:10.1139/g93-024]
7. Asadi Monfared, M., Samsampour, D., Sharifi-Sirchi, G.R., Sadeghi, F. (2018). Assessment of genetic diversity in Salvadora persica L. based on inter simple sequence repeat (ISSR) genetic marker. Journal of Genetic Engineering and Biotechnology, 16, 661-667. [DOI:10.1016/j.jgeb.2018.04.005]
8. Baruah, J., Gogoi, B., Das, K., Ahmed, N.M., Sarmah, D.K., Lal, M., Bhau, B.S. (2017). Genetic diversity study amongst Cymbopogon species from NE-India using RAPD and ISSR markers. Industrial Crops and Products, 95, 235-243. [DOI:10.1016/j.indcrop.2016.10.022]
9. Bettencourt, S.X., Mendonça, D., Lopes, M.S., Rocha, S., Monjardino, P., Monteiro, L., Machado, A.deC. (2015). Genetic diversity and population structure of the endemic Azorean juniper, Juniperus brevifolia (Seub.) Antoine, inferred from SSRs and ISSR markers. Biochemical Systematics and Ecology, 59, 314 - 324. [DOI:10.1016/j.bse.2015.02.003]
10. Bhattarai, S., Chaudhary, R.P., Taylor, R.S.L. (2007). Plants used as fence and fuelwood in manang district, Central Nepal. The Scientific World Journal, 5(5), 107-111. [DOI:10.3126/sw.v5i5.2666]
11. Botstein, D., White, R.L., Skolnick, M., Davis, R.W. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32(3), 314-331.
12. Ciesla, W.M. (2002). Juniper forests - a special challenge for sustainable forestry. Forests, Trees and Livelihoods, 12(3), 195-197. [DOI:10.1080/14728028.2002.9752424]
13. Farjon, A. (2005). A monograph of Cupressaceae and Sciadopitys. Royal Botanic Gardens, London - Kew. 648 p.
14. Farjon, A. (2001). World Checklist and Bibliography of Conifers (2nd Ed). Royal Botanic Gardens, London - Kew
15. Feng, X., Liu, J., Zhang, Y., Wu, W., Pan, Y., Wang, D., Li, D. (2020). Podophyllotoxin profiles combined with SRAP molecular markers in Juniperus rigida: A promising alternative source of podophyllotoxin. Industrial Crops and Products, 153, 112547. [DOI:10.1016/j.indcrop.2020.112547]
16. Füssel, H.M. (2010). Review and quantitative analysis of indices of climate change exposure, adaptive capacity, sensitivity, and impacts. Potsdam Institute for Climate Impact Research (PIK), World Development Report, Germany. 1-35.
17. Ge, X.J., Zhou, X.L., Li, Z.C., Hsu, T.W., Schaal, B.A., Chiang, T.Y. (2005). Low genetic diversity and significant population structuring in the relict Amentotaxus argotaenia complex (Taxaceae) based on ISSR fingerprinting. Journal of Plant Research, 118, 415-422. [DOI:10.1007/s10265-005-0235-1]
18. Ghislain, M., Zhang, D., Fajardo, D., Huamann, Z., Hijmans, R. (1999). Marker-assisted sampling of the cultivated Andean potato Solanum phurejacollection using RAPD markers. Genetic Resources and Crop Evolution, 46, 547-555. [DOI:10.1023/A:1008724007888]
19. Giraldo, R., Caballero, W., Camacho-Tamayo, J. (2018). Mantel test for spatial functional data. AStA Advances in Statistical Analysis, 102, 21-39. [DOI:10.1007/s10182-016-0280-1]
20. Govindaraj, M., Vetriventhan, M., Srinivasan, M. (2015). Importance of Genetic Diversity Assessment in Crop Plants and Its Recent Advances: An Overview of Its Analytical Perspectives. Genetics Research International, ID 431487. [DOI:10.1155/2015/431487]
21. Guizado, S.J.V., Nadeem, M.A., Ali, F., Barut, M., Habyarimana, E., Gómez, T.P., Santillan, J.A.V., Canales, E.T., Gómez, J.C.C., Chung, G., Baloch, F.S. (2020). Genetic diversity and population structure of endangered rosewood from the Peruvian Amazon using ISSR markers. Acta Amazonica, 50(3), 204-212. [DOI:10.1590/1809-4392201902203]
22. Hamrick, J.L. (1987). Gene Flow and Distribution of Genetic Variation in Plant populations / Urbanska K. Differentiation Patterns in Higher Plants. New York - Academic Press.
23. Hojjati, F., Kazempour-Osaloo, S., Adams, R.P., Assadi, M. (2018). Molecular phylogeny of Juniperus in Iran with special reference to the J. excelsa complex, focusing on J. seravschanica. Phytotaxa, 375 (2), 135-157. [DOI:10.11646/phytotaxa.375.2.1]
24. Johnsen, T.N. (1962). One-seed Juniper invasion of northern Arizona grasslands. Ecological Monographs, 32, 187-207. [DOI:10.2307/1942398]
25. Khantemirova, E.V. Semerikov, V.L. (2010). Genetic variation of some varieties of common juniper Juniperus communis L. inferred from analysis of allozyme loci. Russian Journal of Genetics, 46 (5), 546-554. [DOI:10.1134/S1022795410050066]
26. Kling, M.M., Ackerly, D.D. (2021). Global wind patterns shape genetic differentiation, asymmetric gene flow, and genetic diversity in trees. PNAS, 118 (17). e2017317118. [DOI:10.1073/pnas.2017317118]
27. Kumla, S., Doolgindachbaporn, S., Sudmoon, R., Sattayasai, N. (2012). Genetic variation, population structure and identification of yellow catfish, Mystus nemurus (C&V) in Thailand using RAPD, ISSR and SCAR marker. Molecular Biology Reports, 39, 5201-5210. [DOI:10.1007/s11033-011-1317-x]
28. Liu, H., Hu, S., Dong, L., Zhang, H., Zeng, Y., Yu, W., Dai, W., (2014). SRAP analysis of genetic diversity of four female populations in Torreya grandis. Nonwood Forest Research, 3, 30-35. (In Chinese)
29. Liu, X., Du, J., Khan, M.D.A., Cheng, J., Wei, C., Mei, Z., Chen, H., He, T., Fu, J. (2020). Analysis of genetic diversity and similarities between different Lycium varieties based on ISSR analysis and RAMP‑PCR markers. World Academy of Sciences Journal, 2, 83-90. [DOI:10.3892/wasj.2020.39]
30. Lodhi, M.A., Ye, G.N., Weeden, N.F., Reisch, B.I. (1994). A Simple and Efficient Method for DNA Extraction from Grapevine Cultivars and Vitis Species. Plant Molecular Biology Reporter, 12 (1), 6-13. [DOI:10.1007/BF02668658]
31. Mao, K., Hao, G., Liu, J., Adams, R.P., Milne, R.I. (2010). Diversification and biogeography of Juniperus (Cupressaceae): variable diversification rates and multiple intercontinental dispersals. New Phytologist, 188 (1), 254-272. [DOI:10.1111/j.1469-8137.2010.03351.x]
32. Miller, A., Cope, T. (1996). Flora of the Arabian Peninsula and Socotra, vol. 1. Edinburgh - Edinburgh University Press,
33. Mir, M.A., Mansoor, S., Sugapriya, M., Alyemeni, M.N., Wijaya, L., Ahmad, P. (2021). Deciphering genetic diversity analysis of saffron (Crocus sativus L.) using RAPD and ISSR markers. Saudi Journal of Biological Sciences, 28, 1308-1317. [DOI:10.1016/j.sjbs.2020.11.063]
34. Nei, M. (1987). Molecular Evolutionary Genetics. New York - Columbia University Press. [DOI:10.7312/nei-92038]
35. Owens, J.N., Takaso, T., R:union:s, C.J. (1998). Pollination in conifers. Trends in Plant Science, 3(12), 479-485. [DOI:10.1016/S1360-1385(98)01337-5]
36. Potter, L.D., Rowley, J. (1960). Pollen rain and vegetation, San Augustin Plains, New Mexico. Botanical Gazette, 112, 1-25. [DOI:10.1086/336081]
37. Powell, W., Machray, G.C., Provan, J. (1996). Polymorphism revealed by simple sequence repeats. Trends in Plant Science, 1, 215-222. [DOI:10.1016/S1360-1385(96)86898-0]
38. Prevost, A., Wilkinson, M.J. (1999). A new system of comparing PCR primers appliedto ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics, 98, 107-112. [DOI:10.1007/s001220051046]
39. Qin, A., Ding, Y., Jian, Z., Ma, F., Worth, J.R.P., Pei, S., Xu, G., Guo, Q., Shi, Z. (2021). Low genetic diversity and population differentiation in Thuja sutchuenensis Franch., an extremely endangered rediscovered conifer species in southwestern China. Global Ecology and Conservation, 25, e01430. [DOI:10.1016/j.gecco.2020.e01430]
40. Rajwade, A.V., Arora, R.S., Kadoo, N.Y., Harsulkar, A.M., Ghorpade, P.B., Gupta, V.S. (2010). Relatedness of Indian flax Genotypes (Linum usitatissimum L.): An inter-simple sequence repeat (ISSR) primer assay. Molecular Biotechnology, 45, 161-170. [DOI:10.1007/s12033-010-9256-7]
41. Reche, J., García-Martínez, S., Carbonell, P., Almansa, M.S., Hernández, F., Legua, P., Amorós, A. (2019). Relationships between physico-chemical and functional parameters and genetic analysis with ISSR markers in Spanish jujubes (Ziziphus jujuba Mill.) cultivars. Scientia Horticulturae, 253, 390-398. [DOI:10.1016/j.scienta.2019.04.068]
42. Reim, S., Lochschmidt, F., Proft, A., Tröber, U., Wolf, H. (2016). Genetic structure and diversity in Juniperus communis populations in Saxony, Germany. Biodiversity; Research and Conservation, 42, 9-18. [DOI:10.1515/biorc-2016-0008]
43. Rohlf, F.J. (2009). NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Version 2.2. Exeter Software, New York - Applied Biostatistics Inc.
44. Rumeu, B., Vargas, P., Molina, R., Nogales, M., Caujapé-Castells, J., (2014). Phylogeography and genetic structure of the threatened Canarian Junipeurs cedrus (Cupressaceae). Botanical Journal of the Linnean Society, 175 (3), 376e394. [DOI:10.1111/boj.12172]
45. Shahabfar, A., Eitzinger, J. (2013). Spatio-Temporal Analysis of Droughts in Semi-Arid Regions by Using Meteorological Drought Indices. Atmosphere, 4, 94-112. [DOI:10.3390/atmos4020094]
46. Sharma, H.K., Sarkar, M., Choudhary, S.B., Kumar, A.A., Maruthi, R.T., Mitra, J., Karmakar, P.G. (2016). Diversity analysis based on agro-morphological traits and microsatellite based markers in global germplasm collections of roselle (Hibiscus sabdariffa L.). Industrial Crops and Products, 89, 303-315. [DOI:10.1016/j.indcrop.2016.05.027]
47. Su, Y, Wang, T., Ouyang, P. (2009). High genetic differentiation and variation as revealed by ISSR marker in Pseudotaxus chienii (Taxaceae), an old rare conifer endemic to China. Biochemical Systematics and Ecology, 37, 579-588. [DOI:10.1016/j.bse.2009.10.005]
48. Tikendra, L., Amom, T., Nongdam, P. (2019). Molecular genetic homogeneity assessment of micropropagated Dendrobium moschatum Sw. - A rare medicinal orchid, using RAPD and ISSR markers. Plant Gene, 19, 100196. [DOI:10.1016/j.plgene.2019.100196]
49. Varshney, R.K., Chabane, K., Hendre, P.S., Aggarwal, R.K., Graner, A. (2007). Comparative assessment of EST-SSR EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys. Plant Science, 173, 638-649. [DOI:10.1016/j.plantsci.2007.08.010]
50. Williams, J.W., Jackson, S.T., Kutzbach, J.E. (2007). Projected distributions of novel and disappearing climates by 2100 AD. PNAS, 104 (14), 5738-5742. [DOI:10.1073/pnas.0606292104]
51. Yan, W., Li, J., Zheng, D., Friedman, C., Wang, H. (2019). Analysis of genetic population structure and diversity in Mallotus oblongifolius using ISSR and SRAP markers. PeerJ, 7, e7173. [DOI:10.7717/peerj.7173]
52. Zhang, Z.Y., Chen, Y.Y., Li, D.Z. (2005). Detection of low genetic variation in a critically endangered Chinese pine, Pinus squamata, using RAPD and ISSR markers. Biochemical Genetics, 43, 239-249. [DOI:10.1007/s10528-005-5215-6]
53. Adams, R.P. (2004). Junipers of the World: the genus Juniperus. Vancouver, BC - Trafford.
54. Adams, R.P. (2008). Junipers of the World: the genus Juniperus (2nd Ed), Vancouver, BC - Trafford.
55. Adams, R.P. (2014). Junipers of the world: The genus Juniperus (3rd Ed). Victoria, BC - Trafford.
56. Adams, R.P., Al-Farsi, A., Schwarzback, A. (2014). Confirmation of the southernmost population of Juniperus seravschanica in Oman by DNA sequencing of nrDNA and four cpDNA regions. Phytologia, 96 (3), 218-224.
57. Al-Turki. T.A., Basahi, M.A. (2015). Assessment of ISSR based molecular genetic diversity of Hassawi rice in Saudi Arabia. Saudi Journal of Biological Sciences, 22, 591-599. [DOI:10.1016/j.sjbs.2015.06.027]
58. Anderson, J.A., Churchill, G.A., Autrique, J.E., Tanksley, S.D., Sorrells, M.E. (1993). Optimizing parental selection for genetic linkage maps. Genome, 36(1), 181-186. [DOI:10.1139/g93-024]
59. Asadi Monfared, M., Samsampour, D., Sharifi-Sirchi, G.R., Sadeghi, F. (2018). Assessment of genetic diversity in Salvadora persica L. based on inter simple sequence repeat (ISSR) genetic marker. Journal of Genetic Engineering and Biotechnology, 16, 661-667. [DOI:10.1016/j.jgeb.2018.04.005]
60. Baruah, J., Gogoi, B., Das, K., Ahmed, N.M., Sarmah, D.K., Lal, M., Bhau, B.S. (2017). Genetic diversity study amongst Cymbopogon species from NE-India using RAPD and ISSR markers. Industrial Crops and Products, 95, 235-243. [DOI:10.1016/j.indcrop.2016.10.022]
61. Bettencourt, S.X., Mendonça, D., Lopes, M.S., Rocha, S., Monjardino, P., Monteiro, L., Machado, A.deC. (2015). Genetic diversity and population structure of the endemic Azorean juniper, Juniperus brevifolia (Seub.) Antoine, inferred from SSRs and ISSR markers. Biochemical Systematics and Ecology, 59, 314 - 324. [DOI:10.1016/j.bse.2015.02.003]
62. Bhattarai, S., Chaudhary, R.P., Taylor, R.S.L. (2007). Plants used as fence and fuelwood in manang district, Central Nepal. The Scientific World Journal, 5(5), 107-111. [DOI:10.3126/sw.v5i5.2666]
63. Botstein, D., White, R.L., Skolnick, M., Davis, R.W. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32(3), 314-331.
64. Ciesla, W.M. (2002). Juniper forests - a special challenge for sustainable forestry. Forests, Trees and Livelihoods, 12(3), 195-197. [DOI:10.1080/14728028.2002.9752424]
65. Farjon, A. (2005). A monograph of Cupressaceae and Sciadopitys. Royal Botanic Gardens, London - Kew. 648 p.
66. Farjon, A. (2001). World Checklist and Bibliography of Conifers (2nd Ed). Royal Botanic Gardens, London - Kew
67. Feng, X., Liu, J., Zhang, Y., Wu, W., Pan, Y., Wang, D., Li, D. (2020). Podophyllotoxin profiles combined with SRAP molecular markers in Juniperus rigida: A promising alternative source of podophyllotoxin. Industrial Crops and Products, 153, 112547. [DOI:10.1016/j.indcrop.2020.112547]
68. Füssel, H.M. (2010). Review and quantitative analysis of indices of climate change exposure, adaptive capacity, sensitivity, and impacts. Potsdam Institute for Climate Impact Research (PIK), World Development Report, Germany. 1-35.
69. Ge, X.J., Zhou, X.L., Li, Z.C., Hsu, T.W., Schaal, B.A., Chiang, T.Y. (2005). Low genetic diversity and significant population structuring in the relict Amentotaxus argotaenia complex (Taxaceae) based on ISSR fingerprinting. Journal of Plant Research, 118, 415-422. [DOI:10.1007/s10265-005-0235-1]
70. Ghislain, M., Zhang, D., Fajardo, D., Huamann, Z., Hijmans, R. (1999). Marker-assisted sampling of the cultivated Andean potato Solanum phurejacollection using RAPD markers. Genetic Resources and Crop Evolution, 46, 547-555. [DOI:10.1023/A:1008724007888]
71. Giraldo, R., Caballero, W., Camacho-Tamayo, J. (2018). Mantel test for spatial functional data. AStA Advances in Statistical Analysis, 102, 21-39. [DOI:10.1007/s10182-016-0280-1]
72. Govindaraj, M., Vetriventhan, M., Srinivasan, M. (2015). Importance of Genetic Diversity Assessment in Crop Plants and Its Recent Advances: An Overview of Its Analytical Perspectives. Genetics Research International, ID 431487. [DOI:10.1155/2015/431487]
73. Guizado, S.J.V., Nadeem, M.A., Ali, F., Barut, M., Habyarimana, E., Gómez, T.P., Santillan, J.A.V., Canales, E.T., Gómez, J.C.C., Chung, G., Baloch, F.S. (2020). Genetic diversity and population structure of endangered rosewood from the Peruvian Amazon using ISSR markers. Acta Amazonica, 50(3), 204-212. [DOI:10.1590/1809-4392201902203]
74. Hamrick, J.L. (1987). Gene Flow and Distribution of Genetic Variation in Plant populations / Urbanska K. Differentiation Patterns in Higher Plants. New York - Academic Press.
75. Hojjati, F., Kazempour-Osaloo, S., Adams, R.P., Assadi, M. (2018). Molecular phylogeny of Juniperus in Iran with special reference to the J. excelsa complex, focusing on J. seravschanica. Phytotaxa, 375 (2), 135-157. [DOI:10.11646/phytotaxa.375.2.1]
76. Johnsen, T.N. (1962). One-seed Juniper invasion of northern Arizona grasslands. Ecological Monographs, 32, 187-207. [DOI:10.2307/1942398]
77. Khantemirova, E.V. Semerikov, V.L. (2010). Genetic variation of some varieties of common juniper Juniperus communis L. inferred from analysis of allozyme loci. Russian Journal of Genetics, 46 (5), 546-554. [DOI:10.1134/S1022795410050066]
78. Kling, M.M., Ackerly, D.D. (2021). Global wind patterns shape genetic differentiation, asymmetric gene flow, and genetic diversity in trees. PNAS, 118 (17). e2017317118. [DOI:10.1073/pnas.2017317118]
79. Kumla, S., Doolgindachbaporn, S., Sudmoon, R., Sattayasai, N. (2012). Genetic variation, population structure and identification of yellow catfish, Mystus nemurus (C&V) in Thailand using RAPD, ISSR and SCAR marker. Molecular Biology Reports, 39, 5201-5210. [DOI:10.1007/s11033-011-1317-x]
80. Liu, H., Hu, S., Dong, L., Zhang, H., Zeng, Y., Yu, W., Dai, W., (2014). SRAP analysis of genetic diversity of four female populations in Torreya grandis. Nonwood Forest Research, 3, 30-35. (In Chinese)
81. Liu, X., Du, J., Khan, M.D.A., Cheng, J., Wei, C., Mei, Z., Chen, H., He, T., Fu, J. (2020). Analysis of genetic diversity and similarities between different Lycium varieties based on ISSR analysis and RAMP‑PCR markers. World Academy of Sciences Journal, 2, 83-90. [DOI:10.3892/wasj.2020.39]
82. Lodhi, M.A., Ye, G.N., Weeden, N.F., Reisch, B.I. (1994). A Simple and Efficient Method for DNA Extraction from Grapevine Cultivars and Vitis Species. Plant Molecular Biology Reporter, 12 (1), 6-13. [DOI:10.1007/BF02668658]
83. Mao, K., Hao, G., Liu, J., Adams, R.P., Milne, R.I. (2010). Diversification and biogeography of Juniperus (Cupressaceae): variable diversification rates and multiple intercontinental dispersals. New Phytologist, 188 (1), 254-272. [DOI:10.1111/j.1469-8137.2010.03351.x]
84. Miller, A., Cope, T. (1996). Flora of the Arabian Peninsula and Socotra, vol. 1. Edinburgh - Edinburgh University Press,
85. Mir, M.A., Mansoor, S., Sugapriya, M., Alyemeni, M.N., Wijaya, L., Ahmad, P. (2021). Deciphering genetic diversity analysis of saffron (Crocus sativus L.) using RAPD and ISSR markers. Saudi Journal of Biological Sciences, 28, 1308-1317. [DOI:10.1016/j.sjbs.2020.11.063]
86. Nei, M. (1987). Molecular Evolutionary Genetics. New York - Columbia University Press. [DOI:10.7312/nei-92038]
87. Owens, J.N., Takaso, T., R:union:s, C.J. (1998). Pollination in conifers. Trends in Plant Science, 3(12), 479-485. [DOI:10.1016/S1360-1385(98)01337-5]
88. Potter, L.D., Rowley, J. (1960). Pollen rain and vegetation, San Augustin Plains, New Mexico. Botanical Gazette, 112, 1-25. [DOI:10.1086/336081]
89. Powell, W., Machray, G.C., Provan, J. (1996). Polymorphism revealed by simple sequence repeats. Trends in Plant Science, 1, 215-222. [DOI:10.1016/S1360-1385(96)86898-0]
90. Prevost, A., Wilkinson, M.J. (1999). A new system of comparing PCR primers appliedto ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics, 98, 107-112. [DOI:10.1007/s001220051046]
91. Qin, A., Ding, Y., Jian, Z., Ma, F., Worth, J.R.P., Pei, S., Xu, G., Guo, Q., Shi, Z. (2021). Low genetic diversity and population differentiation in Thuja sutchuenensis Franch., an extremely endangered rediscovered conifer species in southwestern China. Global Ecology and Conservation, 25, e01430. [DOI:10.1016/j.gecco.2020.e01430]
92. Rajwade, A.V., Arora, R.S., Kadoo, N.Y., Harsulkar, A.M., Ghorpade, P.B., Gupta, V.S. (2010). Relatedness of Indian flax Genotypes (Linum usitatissimum L.): An inter-simple sequence repeat (ISSR) primer assay. Molecular Biotechnology, 45, 161-170. [DOI:10.1007/s12033-010-9256-7]
93. Reche, J., García-Martínez, S., Carbonell, P., Almansa, M.S., Hernández, F., Legua, P., Amorós, A. (2019). Relationships between physico-chemical and functional parameters and genetic analysis with ISSR markers in Spanish jujubes (Ziziphus jujuba Mill.) cultivars. Scientia Horticulturae, 253, 390-398. [DOI:10.1016/j.scienta.2019.04.068]
94. Reim, S., Lochschmidt, F., Proft, A., Tröber, U., Wolf, H. (2016). Genetic structure and diversity in Juniperus communis populations in Saxony, Germany. Biodiversity; Research and Conservation, 42, 9-18. [DOI:10.1515/biorc-2016-0008]
95. Rohlf, F.J. (2009). NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Version 2.2. Exeter Software, New York - Applied Biostatistics Inc.
96. Rumeu, B., Vargas, P., Molina, R., Nogales, M., Caujapé-Castells, J., (2014). Phylogeography and genetic structure of the threatened Canarian Junipeurs cedrus (Cupressaceae). Botanical Journal of the Linnean Society, 175 (3), 376e394. [DOI:10.1111/boj.12172]
97. Shahabfar, A., Eitzinger, J. (2013). Spatio-Temporal Analysis of Droughts in Semi-Arid Regions by Using Meteorological Drought Indices. Atmosphere, 4, 94-112. [DOI:10.3390/atmos4020094]
98. Sharma, H.K., Sarkar, M., Choudhary, S.B., Kumar, A.A., Maruthi, R.T., Mitra, J., Karmakar, P.G. (2016). Diversity analysis based on agro-morphological traits and microsatellite based markers in global germplasm collections of roselle (Hibiscus sabdariffa L.). Industrial Crops and Products, 89, 303-315. [DOI:10.1016/j.indcrop.2016.05.027]
99. Su, Y, Wang, T., Ouyang, P. (2009). High genetic differentiation and variation as revealed by ISSR marker in Pseudotaxus chienii (Taxaceae), an old rare conifer endemic to China. Biochemical Systematics and Ecology, 37, 579-588. [DOI:10.1016/j.bse.2009.10.005]
100. Tikendra, L., Amom, T., Nongdam, P. (2019). Molecular genetic homogeneity assessment of micropropagated Dendrobium moschatum Sw. - A rare medicinal orchid, using RAPD and ISSR markers. Plant Gene, 19, 100196. [DOI:10.1016/j.plgene.2019.100196]
101. Varshney, R.K., Chabane, K., Hendre, P.S., Aggarwal, R.K., Graner, A. (2007). Comparative assessment of EST-SSR EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys. Plant Science, 173, 638-649. [DOI:10.1016/j.plantsci.2007.08.010]
102. Williams, J.W., Jackson, S.T., Kutzbach, J.E. (2007). Projected distributions of novel and disappearing climates by 2100 AD. PNAS, 104 (14), 5738-5742. [DOI:10.1073/pnas.0606292104]
103. Yan, W., Li, J., Zheng, D., Friedman, C., Wang, H. (2019). Analysis of genetic population structure and diversity in Mallotus oblongifolius using ISSR and SRAP markers. PeerJ, 7, e7173. [DOI:10.7717/peerj.7173]
104. Zhang, Z.Y., Chen, Y.Y., Li, D.Z. (2005). Detection of low genetic variation in a critically endangered Chinese pine, Pinus squamata, using RAPD and ISSR markers. Biochemical Genetics, 43, 239-249. [DOI:10.1007/s10528-005-5215-6]
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Rahimian Boogar A, Salehi H. ISSR-based genetic diversity assessment of five populations of Juniperus polycarpos K. Koch in southern habitats of Iran. FOP. 2021; 6 (1) :1-12
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گل و گیاهان زینتی Flower and Ornamental Plants
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