RT - Journal Article T1 - In vitro organogenesis of Salep (Orchis mascula L.) JF - flowerjournal YR - 2020 JO - flowerjournal VO - 5 IS - 1 UR - http://flowerjournal.ir/article-1-178-en.html SP - 19 EP - 24 K1 - Orchis mascula L. K1 - In vitro culture K1 - Shoot induction K1 - Root induction AB - Orchis muscula L. is one of the worthwhile ornamental, medicinal, native and endangered species in Iran. In this research, plant tissue culture method has been used to propagate this valuable plant. For in vitro organogenesis, protocorm explants were surface sterilized and then cultured on different shoot and root induction media based on OrchiMax (OM) medium, supplemented with plant growth regulators with different concentrations alone or in combination. Based on results, the most shoot induction (100%) was obtained from protocorm explants cultured on medium containing 2 mg L-1 BAP and 0.5 mg L-1 NAA and the highest root induction (80%) was belonged to the treatment of IAA at 1 mgL-1. LA eng UL http://flowerjournal.ir/article-1-178-en.html M3 10.52547/flowerjournal.5.1.19 ER -