:: Volume 5, Issue 1 (Spring and Summer 2020) ::
FOP 2020, 5(1): 1-12 Back to browse issues page
Direct organogenesis from different vegetative explants of Gerbera jamesonii cv. Nilo
Nima Ahmadi
Abstract:   (2511 Views)
Developing a highly efficient protocol for plant regeneration is a prerequisite for successful gene transformation in plants. To gain these properties, several experiments were conducted on various explants to optimize the concentration of plant growth regulators in regeneration of Gerbera. In the first experiment, regeneration of Gerbera plant was investigated using different explants including intact leaf, petiole, scratched petiole and Thin Cell Layer (TCL). Moreover, combination of BAP (0, 1, 2 and 3 mg L-1), TDZ (0, 0.1, 0.5, 0.8 and 1 mg L-1) and IAA (0.1 mg L-1) under 16/8 h light/darkness condition or one-month darkness were considered. In the second experiment, the regeneration potential of the first, second and third disc of TCL cut off from the end of the detached petioles were evaluated. Effect of different combination of BAP (0, 1 and 2 mg L-1), TDZ (0 and 0.5 mg L-1) and IAA (0, 0.1 and 0.3 mg L-1) on regeneration was studied. In all experiments, the MS-basal medium was used. The results showed that the best regeneration medium for intact leaf (33.2% regeneration, 66.3 shoots), petiole (86.66% regeneration, 33.33 shoots) and scratched petiole (53.2% regeneration, 33.10 shoots) was MS medium containing 2 mg L-1 BAP under 16/8 h light/darkness condition, and for TCL explants (46% regeneration, 29 shoots), 2 mg L-1 BAP with 0.5 mg L-1 TDZ in the darkness. In the second experiment, the highest rate of regeneration was obtained from the first disc of TCL explants on the MS medium containing 2 mg L-1 BAP, 0.5 mg L-1 TDZ and 0.1 mg L-1 IAA.
Keywords: Organogenesis, Plant growth regulators, Regeneration, Thin Cell Layer
Full-Text [PDF 852 kb]   (567 Downloads)    
Type of Study: Research | Subject: Special
Received: 2019/11/11 | Accepted: 2021/01/7 | Published: 2021/05/3
References
1. Altaf, N., Khan, A.R., Ali, L., Bhatti, I.A. (2009). Tissue culture of gerbera. Pakistan Journal of Botany, 41(1), 7-10.
2. Azadi, P., Bagheri, H., Nalousi, A.M., Nazari, F., Chandler, S.F. (2016). Current status and biotechnological advances in genetic engineering of ornamental plants. Biotechnology Advances, 34(6), 1073-1090. [DOI:10.1016/j.biotechadv.2016.06.006]
3. Bhatia, R., Singh, K., Jhang, T., Sharma, T. (2009). Assessment of clonal fidelity of micropropagated gerbera plants by ISSR markers. Scientia Horticulturae, 119(2), 208-211. [DOI:10.1016/j.scienta.2008.07.024]
4. Cardoso, J.C., da Silva, J.A.T. (2013). Gerbera micropropagation. Biotechnology Advances, 31(8), 1344-1357. [DOI:10.1016/j.biotechadv.2013.05.008]
5. Chakrabarty, D., Datta, S.K. (2008). Micropropagation of gerbera, lipid peroxidation and antioxidant enzyme activities during acclimatization process. Acta Physiologiae Plantarum, 30(3), 325-331. [DOI:10.1007/s11738-007-0125-3]
6. Chung, M.-Y., Kim, M.B., Chung, Y.M., Nou, I.-S., Kim, C.K. (2016). In vitro shoot regeneration and genetic transformation of the gerbera (Gerbera hybrida Hort.) cultivar 'Gold Eye'. Journal of Plant Biotechnology, 43(2), 255-260. [DOI:10.5010/JPB.2016.43.2.255]
7. Elomaa, P., Honkanen, J., Puska, R., Seppänen, P., Helariutta, Y., Mehto, M., Kotilainen, M., Nevalainen, L., Teeri, T.H. (1993). Agrobacterium-mediated transfer of antisense chalcone synthase cDNA to Gerbera hybrida inhibits flower pigmentation. Nature Biotechnology, 11(4), 508-511. [DOI:10.1038/nbt0493-508]
8. Elomaa, P., Uimari, A., Mehto, M., Albert, V.A., Laitinen, R.A., Teeri, T.H. (2003) Activation of anthocyanin biosynthesis in Gerbera hybrida (Asteraceae) suggests conserved protein-protein and protein-promoter interactions between the anciently diverged monocots and eudicots. Plant Physiology, 133(4), 1831-1842. [DOI:10.1104/pp.103.026039]
9. Huang, M.C., Chu, C.Y. (1985). A scheme for commercial multiplication of Gerbera (Gerbera hybrida Hort.) through shoot tip culture. Journal of the Japanese Society for Horticultural Science, 54(1), 94-100. [DOI:10.2503/jjshs.54.94]
10. Jauhar, P.P. (2006). Modern biotechnology as an integral supplement to conventional plant breeding: the prospects and challenges. Crop Science, 46(4), 1841-1859. [DOI:10.2135/cropsci2005.07-0223]
11. Korbin, M., Podwyszynska, M., Komorowska, B., Wawrzynczak, D. 2001. Transformation of Gerbera plants with Tomato spotted wilt virus (TSWV) nucleoprotein gene. Paper presented at the XX International Eucarpia Symposium, Section Ornamentals, Strategies for New Ornamentals-Part II 572. [DOI:10.17660/ActaHortic.2002.572.17]
12. Meyer, H., Van Staden, J. (1988). The in vitro culture of Gerbera aurantiaca. Plant Cell, Tissue and Organ Culture, 14(1), 25-30. [DOI:10.1007/BF00029572]
13. Murashige, T., Serpa, M., Jones, J.B. (1974). Clonal multiplication of Gerbera through tissue culture. Horticultural Science, 9, 175-180.
14. Nhut, D.T., An, T.T.T., Huong, N.T.D., Don, N.T., Hai, N.T., Thien, N.Q., Vu, N.H. (2007). Effect of genotype, explant size, position, and culture medium on shoot generation of Gerbera jamesonii by receptacle transverse thin cell layer culture. Scientia Horticulturae, 111(2), 146-151. [DOI:10.1016/j.scienta.2006.10.008]
15. Nowak, E., Rojek, Z., Kucharska, D., Orlikowska, T. (1997). Effect of initial explant on effectivity of Agrobacterium tumefaciens-mediated transformation of Gerbera hybrida. Biotechnologia, 4, 27-37.
16. Orlikowska, T., Nowak, E., Marasek, A., Kucharska, D. (1999). Effects of growth regulators and incubation period on in vitro regeneration of adventitious shoots from gerbera petioles. Plant Cell, Tissue and Organ Culture, 59(2), 95-102. [DOI:10.1023/A:1006453905086]
17. Reddy, A.C., Choudhary, M. (2002). Rapid plant regeneration from Gerbera jamesonii Bolus callus cultures. Acta Botanica Croatica, 61(2), 125-134.
18. Reynoird, J.P., Chriqui, D., Noin, M., Brown, S., Marie, D. (1993). Plant regeneration fromin vitro leaf culture of several Gerbera species. Plant Cell, Tissue and Organ Culture, 33(2), 203-210. [DOI:10.1007/BF01983235]
19. Shabanpour, K., Sharifi, A., Bagheri, A., Moshtaghi, N. (2011). Effect of genotypes and culture medium on shoot regeneration and proliferation of Gerbera jamesonii. African Journal of Biotechnology, 10(57), 12211-12217.
20. Sheela, V. (2006). Gerbera. Advances in Ornamental Horticulture, 2, 129-149.
21. Teeri, T.H., Elomaa, P., Kotilainen, M., Albert, V.A. (2006). Mining plant diversity: Gerbera as a model system for plant developmental and biosynthetic research. BioEssays, 28(7), 756-767. [DOI:10.1002/bies.20439]
22. Altaf, N., Khan, A.R., Ali, L., Bhatti, I.A. (2009). Tissue culture of gerbera. Pakistan Journal of Botany, 41(1), 7-10.
23. Azadi, P., Bagheri, H., Nalousi, A.M., Nazari, F., Chandler, S.F. (2016). Current status and biotechnological advances in genetic engineering of ornamental plants. Biotechnology Advances, 34(6), 1073-1090. [DOI:10.1016/j.biotechadv.2016.06.006]
24. Bhatia, R., Singh, K., Jhang, T., Sharma, T. (2009). Assessment of clonal fidelity of micropropagated gerbera plants by ISSR markers. Scientia Horticulturae, 119(2), 208-211. [DOI:10.1016/j.scienta.2008.07.024]
25. Cardoso, J.C., da Silva, J.A.T. (2013). Gerbera micropropagation. Biotechnology Advances, 31(8), 1344-1357. [DOI:10.1016/j.biotechadv.2013.05.008]
26. Chakrabarty, D., Datta, S.K. (2008). Micropropagation of gerbera, lipid peroxidation and antioxidant enzyme activities during acclimatization process. Acta Physiologiae Plantarum, 30(3), 325-331. [DOI:10.1007/s11738-007-0125-3]
27. Chung, M.-Y., Kim, M.B., Chung, Y.M., Nou, I.-S., Kim, C.K. (2016). In vitro shoot regeneration and genetic transformation of the gerbera (Gerbera hybrida Hort.) cultivar 'Gold Eye'. Journal of Plant Biotechnology, 43(2), 255-260. [DOI:10.5010/JPB.2016.43.2.255]
28. Elomaa, P., Honkanen, J., Puska, R., Seppänen, P., Helariutta, Y., Mehto, M., Kotilainen, M., Nevalainen, L., Teeri, T.H. (1993). Agrobacterium-mediated transfer of antisense chalcone synthase cDNA to Gerbera hybrida inhibits flower pigmentation. Nature Biotechnology, 11(4), 508-511. [DOI:10.1038/nbt0493-508]
29. Elomaa, P., Uimari, A., Mehto, M., Albert, V.A., Laitinen, R.A., Teeri, T.H. (2003) Activation of anthocyanin biosynthesis in Gerbera hybrida (Asteraceae) suggests conserved protein-protein and protein-promoter interactions between the anciently diverged monocots and eudicots. Plant Physiology, 133(4), 1831-1842. [DOI:10.1104/pp.103.026039]
30. Huang, M.C., Chu, C.Y. (1985). A scheme for commercial multiplication of Gerbera (Gerbera hybrida Hort.) through shoot tip culture. Journal of the Japanese Society for Horticultural Science, 54(1), 94-100. [DOI:10.2503/jjshs.54.94]
31. Jauhar, P.P. (2006). Modern biotechnology as an integral supplement to conventional plant breeding: the prospects and challenges. Crop Science, 46(4), 1841-1859. [DOI:10.2135/cropsci2005.07-0223]
32. Korbin, M., Podwyszynska, M., Komorowska, B., Wawrzynczak, D. 2001. Transformation of Gerbera plants with Tomato spotted wilt virus (TSWV) nucleoprotein gene. Paper presented at the XX International Eucarpia Symposium, Section Ornamentals, Strategies for New Ornamentals-Part II 572. [DOI:10.17660/ActaHortic.2002.572.17]
33. Meyer, H., Van Staden, J. (1988). The in vitro culture of Gerbera aurantiaca. Plant Cell, Tissue and Organ Culture, 14(1), 25-30. [DOI:10.1007/BF00029572]
34. Murashige, T., Serpa, M., Jones, J.B. (1974). Clonal multiplication of Gerbera through tissue culture. Horticultural Science, 9, 175-180.
35. Nhut, D.T., An, T.T.T., Huong, N.T.D., Don, N.T., Hai, N.T., Thien, N.Q., Vu, N.H. (2007). Effect of genotype, explant size, position, and culture medium on shoot generation of Gerbera jamesonii by receptacle transverse thin cell layer culture. Scientia Horticulturae, 111(2), 146-151. [DOI:10.1016/j.scienta.2006.10.008]
36. Nowak, E., Rojek, Z., Kucharska, D., Orlikowska, T. (1997). Effect of initial explant on effectivity of Agrobacterium tumefaciens-mediated transformation of Gerbera hybrida. Biotechnologia, 4, 27-37.
37. Orlikowska, T., Nowak, E., Marasek, A., Kucharska, D. (1999). Effects of growth regulators and incubation period on in vitro regeneration of adventitious shoots from gerbera petioles. Plant Cell, Tissue and Organ Culture, 59(2), 95-102. [DOI:10.1023/A:1006453905086]
38. Reddy, A.C., Choudhary, M. (2002). Rapid plant regeneration from Gerbera jamesonii Bolus callus cultures. Acta Botanica Croatica, 61(2), 125-134.
39. Reynoird, J.P., Chriqui, D., Noin, M., Brown, S., Marie, D. (1993). Plant regeneration fromin vitro leaf culture of several Gerbera species. Plant Cell, Tissue and Organ Culture, 33(2), 203-210. [DOI:10.1007/BF01983235]
40. Shabanpour, K., Sharifi, A., Bagheri, A., Moshtaghi, N. (2011). Effect of genotypes and culture medium on shoot regeneration and proliferation of Gerbera jamesonii. African Journal of Biotechnology, 10(57), 12211-12217.
41. Sheela, V. (2006). Gerbera. Advances in Ornamental Horticulture, 2, 129-149.
42. Teeri, T.H., Elomaa, P., Kotilainen, M., Albert, V.A. (2006). Mining plant diversity: Gerbera as a model system for plant developmental and biosynthetic research. BioEssays, 28(7), 756-767. [DOI:10.1002/bies.20439]



XML   Persian Abstract   Print



Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 5, Issue 1 (Spring and Summer 2020) Back to browse issues page