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:: Volume 5, Issue 1 (Spring and Summer 2020) ::
FOP 2020, 5(1): 19-24 Back to browse issues page
In vitro organogenesis of Salep (Orchis mascula L.)
Nasim Zarinpanjeh *
Abstract:   (2909 Views)
Orchis muscula L. is one of the worthwhile ornamental, medicinal, native and endangered species in Iran. In this research, plant tissue culture method has been used to propagate this valuable plant. For in vitro organogenesis, protocorm explants were surface sterilized and then cultured on different shoot and root induction media based on OrchiMax (OM) medium, supplemented with plant growth regulators with different concentrations alone or in combination. Based on results, the most shoot induction (100%) was obtained from protocorm explants cultured on medium containing 2 mg L-1 BAP and 0.5 mg L-1 NAA and the highest root induction (80%) was belonged to the treatment of IAA at 1 mgL-1.
Keywords: Orchis mascula L., In vitro culture, Shoot induction, Root induction
Full-Text [PDF 1158 kb]   (865 Downloads)    
Type of Study: Research | Subject: Special
Received: 2020/11/20 | Accepted: 2021/01/21 | Published: 2021/05/3
References
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2. Chugh, S., Guha, S., Rao, U. (2009). Micropropagation of orchids: A review on the potential of different explants. Scientia Horticulturae, 122(4), 507-520. [DOI:10.1016/j.scienta.2009.07.016]
3. Chung, H.G., Chen, J.T., Chang, W.C. (2005). Cytokinins induce direct somatic embryogenesis of Dendrobium 'Chiengmai pink' and subsequent plant regeneration. In Vitro Cellular and Developmental Biology, 41(6), 765-769. [DOI:10.1079/IVP2005702]
4. Chung, H.G., Chen, J.T., Chang, W.C. (2007.) Plant regeneration through direct somatic embryogenesis from leaf explants of Dendrobium. Biologia Plantarum, 51(2): 346-350. [DOI:10.1007/s10535-007-0069-x]
5. Chung, J.P., Huang, C.Y., Dai, T.E. (2010). Spectral effects on embryogenesis and plantlet growth of Oncidium 'Gower Ramsey'. Scientia Horticulturae, 124(4), 511-516. [DOI:10.1016/j.scienta.2010.01.028]
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19. Chen, Y.C., Chang, C., Chang, W.C. (2000). A reliable protocol for plant regeneration from callus culture of Phalaenopsis. In Vitro Cellular and Developmental Biology, 36, 420-423. [DOI:10.1007/s11627-000-0076-5]
20. Chugh, S., Guha, S., Rao, U. (2009). Micropropagation of orchids: A review on the potential of different explants. Scientia Horticulturae, 122(4), 507-520. [DOI:10.1016/j.scienta.2009.07.016]
21. Chung, H.G., Chen, J.T., Chang, W.C. (2005). Cytokinins induce direct somatic embryogenesis of Dendrobium 'Chiengmai pink' and subsequent plant regeneration. In Vitro Cellular and Developmental Biology, 41(6), 765-769. [DOI:10.1079/IVP2005702]
22. Chung, H.G., Chen, J.T., Chang, W.C. (2007.) Plant regeneration through direct somatic embryogenesis from leaf explants of Dendrobium. Biologia Plantarum, 51(2): 346-350. [DOI:10.1007/s10535-007-0069-x]
23. Chung, J.P., Huang, C.Y., Dai, T.E. (2010). Spectral effects on embryogenesis and plantlet growth of Oncidium 'Gower Ramsey'. Scientia Horticulturae, 124(4), 511-516. [DOI:10.1016/j.scienta.2010.01.028]
24. Ebrahimi, A., Asadi, A.A., Rashidi Monfared, S., Sahebi, M., Rezaee, S., Khaledian, Y. (2020). Evaluation of phenotypic diversity of the endangered orchid (Orchis mascula): Emphasizing on breeding, conservation and development. South African Journal of Botany, 132, 1-12. [DOI:10.1016/j.sajb.2020.05.013]
25. Ghorbani, A., Gravendeel, B., Naghibi, F., Boer, H.D. (2014). Wild orchid tuber collection in Iran: a wake-up call for conservation. Biodiversity and Conservation, 23, 2749-2760. [DOI:10.1007/s10531-014-0746-y]
26. Kim, M.S., Kim, J.Y. (2003). Micropropagation of Dendrobium hybrids through shoot tip culture. Acta Horticulturae, doi:10.17660/ActaHortic.2003.624.73. [DOI:10.17660/ActaHortic.2003.624.73]
27. Luo, J.P., Wang, Y., Zha, X.Q., Huang, L. (2008). Micropropagation of Dendrobium densiflorum Lindle. ex Wall. through protocorm-like bodies: effect of plant growth regulators and lanthanoids. Plant Cell Tissue, and Organ Culture, 93, 333-340. [DOI:10.1007/s11240-008-9381-1]
28. Mozaffarian, V. (2013). Trees and Shrubs of Iran. Farhang Moaser Publisher. 1470p. (In Persian).
29. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497. [DOI:10.1111/j.1399-3054.1962.tb08052.x]
30. Murthy, H.N., Pyati, A.H. (2001). Micropropagation of Aerides maculosum Lindl. (Orchidaceae). In Vitro Cellular & Developmental Biology- Plant, 3, 223-226. [DOI:10.1007/s11627-001-0039-5]
31. Parthibhan, S., Rao, M.V., Kumar, T.S. (2015). In vitro regeneration from protocorms in Dendrobium aqueum Lindley - An imperiled orchid. Journal of Genetic Engineering and Biotechnology, 13(2), 227-233. [DOI:10.1016/j.jgeb.2015.07.001]
32. Seeni, S., Latha, P.G. (2000). In vitro multiplication and ecorehabilitation of the endangered blue Vanda. Plant Cell Tissue, and Organ Culture, 61, 1-8. [DOI:10.1023/A:1006444614657]
33. Shahinul Islam, S.M., Bhattacharjee, B. (2015). Plant regeneration through somatic embryogenesis from leaf and root explants of Rhynchostylis retusa (L.) Blume. Applied Biological Research, 17(2), 158-165. [DOI:10.5958/0974-4517.2015.00025.7]
34. Sheelavantmath, S.S., Murthy, H.N., Pyati, A.N., Kumar, H.G.A., Ravishankar, B.V. (2000). In vitro propagation of the endangered orchid, Geodorum densiflorum (Lam.) Schltr. through rhizome section culture. Plant Cell Tissue, and Organ Culture, 60, 151-154. [DOI:10.1023/A:1006426905052]
35. Tsukazaki, H., Mii, M., Tokuhara, K., Ishikawa, K. (2000). Cryopreservation of Doritaenopsis suspension culture by vitrification. Plant Cell Reports, 19, 1160-1164. [DOI:10.1007/s002990000255]
36. Zare, H., Amini, T., Jafari Gorzin, B. (2019). Orchids and their biodiversity in Caspian habitats. Iran Nature, 4, 81-93 (In Persian).
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Zarinpanjeh N. In vitro organogenesis of Salep (Orchis mascula L.). FOP 2020; 5 (1) :19-24
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Volume 5, Issue 1 (Spring and Summer 2020) Back to browse issues page
گل و گیاهان زینتی Flower and Ornamental Plants
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