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جلد 6 شماره 2 صفحات 164-147 برگشت به فهرست نسخه ها
اثر نوع محیط مایه‌زنی در انتقال ژن کیتیناز کایمری به لیسیانتوس (Eustoma grandiflorum [Raf.] Shinn) برای ایجاد مقاومت به بیماری قارچی Fusarium solani
محمد مهدی فخرائی، علیرضا مطلبی آذر، حسن صالحی ، ناصر مهنا ، مصطفی مطلبی
دانشگاه تبریز
چکیده:   (904 مشاهده)
لیسیانتوس در رده ده گل برتر دنیا می‌‌باشد و در سال 2017 رتبه پنجم صادرات و فروش گیاهان زینتی دنیا را به خود اختصاص داده است. ایجاد مقاومت به بیماری‌‌های قارچی به‌‌ویژه فوزاریوم از مهم­ترین هدف­های بهنژادی لیسیانتوس می‌‌باشد. کارهای کمی در مورد بهنژادی برای مقاومت به بیماری‌‌های قارچی در این گیاه زینتی صورت گرفته است؛ از این رو انتقال ژن کیتیناز کایمری به لیسیانتوس دستاورد بزرگی در رسیدن به این مهم خواهد بود. انتقال و بیان ژن کیتیناز در گیاهان سطوح بالای مقاومت به آلودگی قارچی و تاخیر در بروز نشانه­های بیماری را در زمان رویارویی با بیمارگرهای قارچی باعث می­شود. پژوهشگران ثابت کرده­اند که فعالیت کیتیناز کایمری در تخریب دیواره‌‌های قارچ‌‌ها تفاوت قابل توجهی نسبت به کیتیناز Chit42 دارد. هدف از این پژوهش انگیزش مقاومت به بیماری‌‌ قارچی Fusarium solani در لیسیانتوس از راه انتقال ژن کیتیناز کایمری با استفاده از Agrobacterium tumefaciens بود. در این پژوهش اثر انواع محیط کشت MS، MS½ و LB، دو میزان pH 2/5 و 8/5 و همچنین سوکروز با غلظت­های 30 و 15 میلی‌‌گرم بر لیتر و مالتوز 30 میلی­گرم بر لیتر، در محیط مایه زنی مورد ارزیابی قرار گرفت. نتایج نشان داد که تیمار B شامل محیط مایه زنی MS دارای 30 گرم بر لیتر مالتوز با 2/5pH= بهترین پاسخ را در انتقال این ژن به لیسیانتوس داد و در کنار محیط گزینشی سرسخت‌‌تر دارای 100 میلی‌‌گرم بر لیتر کانامایسین با میانگین باززایی 13/11 گیاهچه از هر قطعه برگ به‌‌طور معنی‌‌داری برتر از سایر تیمارها بود. نتایج این پژوهش نشان داد که محیط کشت MS نسبت به محیط کشت LB در محیط مایه زنی انتقال ژن به لیسیانتوس کارآمدتر است و کاهش pH و تغییر منبع کربوهیدارت از سوکروز به مالتوز در محیط مایه زنی کارایی تراریختی این گیاه را افزایش داد. در این پژوهش در طی انتقال ژن کیتیناز کایمری به لیسیانتوس در محیط گزینشی سرسخت‌‌تر، شمار 471 گیاهچه از 45 قطعه برگ، ایجاد شد و از شمار 21 گیاهچه و لاین به‌‌طور تصادفی گزینش شده، شمار 10 لاین به آزمون PCR و 8 لاین به آزمون زیست‌‌سنجی پاسخ مثبت نشان دادند. این نخستین گزارش انتقال ژن کیتیناز کایمری در لیسیانتوس می­باشد.  
واژه‌های کلیدی: آگروباکتریوم، زیست‌‌سنجی، فوزاریوم، لاین تراریخته، مقاومت به بیماری قارچی
متن کامل [PDF 1857 kb]   (118 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: تخصصي
دریافت: 1400/9/20 | پذیرش: 1400/10/1 | انتشار: 1400/12/16
فهرست منابع
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Fakhraei M M, Motallebi Azar A, Salehi H, mahna N, Motallebi M. Effect of the type of inoculation medium on chimeric chitinase gene transfer to lisianthus (Eustoma grandiflorum [Raf.] Shinn) for resistance to the fungal disease Fusarium solani. FOP. 2021; 6 (2) :147-164
URL: http://flowerjournal.ir/article-1-215-fa.html

فخرائی محمد مهدی، مطلبی آذر علیرضا، صالحی حسن، مهنا ناصر، مطلبی مصطفی. اثر نوع محیط مایه‌زنی در انتقال ژن کیتیناز کایمری به لیسیانتوس (Eustoma grandiflorum [Raf.] Shinn) برای ایجاد مقاومت به بیماری قارچی Fusarium solani. گل و گیاهان زینتی. 1400; 6 (2) :164-147

URL: http://flowerjournal.ir/article-1-215-fa.html



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گل و گیاهان زینتی Flower and Ornamental Plants
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