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:: Volume 6, Issue 1 (Spring and Summer 2021) ::
FOP 2021, 6(1): 1-12 Back to browse issues page
ISSR-based genetic diversity assessment of five populations of Juniperus polycarpos K. Koch in southern habitats of Iran
Abdolrahman Rahimian Boogar * , Hassan Salehi
Department of Horticultural Science and landscape Engineering, Faculty of Agriculture, University of Zabol, Zabol, Iran (Present address).
Abstract:   (2247 Views)
Juniperus polycarpos K. Koch is a durable conifer species with different growth habit that grow in ecological landscapes. In this study, genetic diversity was investigated for five populations of juniper that grew in Fasa, Sepidan, Khabr, Rabor, and Genow habitat in the south of Iran. For DNA extraction, leaf samples of 10 diverse accessions of each habitat were collected (totally 50 accessions). Populations genetic diversity was evaluated based on 12 inter simple sequence repeat (ISSR) markers. Assessment of ISSR markers predicted 75 loci with 67 (89.33%) polymorphic loci, polymorphic information content (PIC) 0.45, resolving power (Rp) 3.78, and effective multiplex ratio (EMR) and marker index (MI) of primers were 5.50 and 2.47, respectively. Populations Nei’s genetic diversity showed higher intra-population genetic diversity (0.25) than inter-population genetic diversity (0.13). Results indicated the highest different loci (Na) for Sepidan population (56) and the highest effective alleles (Ne) were obtained in two populations of Sepidan (1.49) and Genow (1.49). Furthermore, the highest gene diversity (H) observed in the population of Sepidan (0.27) and Genow (0.27). The population of Sepidan has the highest Shannon’s information index (I) (0.41) and percentage of polymorphic loci (P) (74.67). Investigated populations showed high total genetic diversity (Ht) (0.38), intra-population genetic diversity (Hs) (0.25), and inter-population genetic diversity (Dst) (0.13), and moderate genetic differentiation among populations (Gst = 0.34). High gene flow (Nm) was obtained between evaluated populations (0.94). Moreover, AMOVA analysis indicated 70% within and 30% among populations genetic variation.
Keywords: Accession, Conifer, Juniper, Molecular markers, Variation
Full-Text [PDF 445 kb]   (753 Downloads)    
Type of Study: Research | Subject: Special
Received: 2021/08/14 | Accepted: 2021/09/22 | Published: 2022/01/15
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60. Baruah, J., Gogoi, B., Das, K., Ahmed, N.M., Sarmah, D.K., Lal, M., Bhau, B.S. (2017). Genetic diversity study amongst Cymbopogon species from NE-India using RAPD and ISSR markers. Industrial Crops and Products, 95, 235-243. [DOI:10.1016/j.indcrop.2016.10.022]
61. Bettencourt, S.X., Mendonça, D., Lopes, M.S., Rocha, S., Monjardino, P., Monteiro, L., Machado, A.deC. (2015). Genetic diversity and population structure of the endemic Azorean juniper, Juniperus brevifolia (Seub.) Antoine, inferred from SSRs and ISSR markers. Biochemical Systematics and Ecology, 59, 314 - 324. [DOI:10.1016/j.bse.2015.02.003]
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63. Botstein, D., White, R.L., Skolnick, M., Davis, R.W. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32(3), 314-331.
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73. Guizado, S.J.V., Nadeem, M.A., Ali, F., Barut, M., Habyarimana, E., Gómez, T.P., Santillan, J.A.V., Canales, E.T., Gómez, J.C.C., Chung, G., Baloch, F.S. (2020). Genetic diversity and population structure of endangered rosewood from the Peruvian Amazon using ISSR markers. Acta Amazonica, 50(3), 204-212. [DOI:10.1590/1809-4392201902203]
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78. Kling, M.M., Ackerly, D.D. (2021). Global wind patterns shape genetic differentiation, asymmetric gene flow, and genetic diversity in trees. PNAS, 118 (17). e2017317118. [DOI:10.1073/pnas.2017317118]
79. Kumla, S., Doolgindachbaporn, S., Sudmoon, R., Sattayasai, N. (2012). Genetic variation, population structure and identification of yellow catfish, Mystus nemurus (C&V) in Thailand using RAPD, ISSR and SCAR marker. Molecular Biology Reports, 39, 5201-5210. [DOI:10.1007/s11033-011-1317-x]
80. Liu, H., Hu, S., Dong, L., Zhang, H., Zeng, Y., Yu, W., Dai, W., (2014). SRAP analysis of genetic diversity of four female populations in Torreya grandis. Nonwood Forest Research, 3, 30-35. (In Chinese)
81. Liu, X., Du, J., Khan, M.D.A., Cheng, J., Wei, C., Mei, Z., Chen, H., He, T., Fu, J. (2020). Analysis of genetic diversity and similarities between different Lycium varieties based on ISSR analysis and RAMP‑PCR markers. World Academy of Sciences Journal, 2, 83-90. [DOI:10.3892/wasj.2020.39]
82. Lodhi, M.A., Ye, G.N., Weeden, N.F., Reisch, B.I. (1994). A Simple and Efficient Method for DNA Extraction from Grapevine Cultivars and Vitis Species. Plant Molecular Biology Reporter, 12 (1), 6-13. [DOI:10.1007/BF02668658]
83. Mao, K., Hao, G., Liu, J., Adams, R.P., Milne, R.I. (2010). Diversification and biogeography of Juniperus (Cupressaceae): variable diversification rates and multiple intercontinental dispersals. New Phytologist, 188 (1), 254-272. [DOI:10.1111/j.1469-8137.2010.03351.x]
84. Miller, A., Cope, T. (1996). Flora of the Arabian Peninsula and Socotra, vol. 1. Edinburgh - Edinburgh University Press,
85. Mir, M.A., Mansoor, S., Sugapriya, M., Alyemeni, M.N., Wijaya, L., Ahmad, P. (2021). Deciphering genetic diversity analysis of saffron (Crocus sativus L.) using RAPD and ISSR markers. Saudi Journal of Biological Sciences, 28, 1308-1317. [DOI:10.1016/j.sjbs.2020.11.063]
86. Nei, M. (1987). Molecular Evolutionary Genetics. New York - Columbia University Press. [DOI:10.7312/nei-92038]
87. Owens, J.N., Takaso, T., R:union:s, C.J. (1998). Pollination in conifers. Trends in Plant Science, 3(12), 479-485. [DOI:10.1016/S1360-1385(98)01337-5]
88. Potter, L.D., Rowley, J. (1960). Pollen rain and vegetation, San Augustin Plains, New Mexico. Botanical Gazette, 112, 1-25. [DOI:10.1086/336081]
89. Powell, W., Machray, G.C., Provan, J. (1996). Polymorphism revealed by simple sequence repeats. Trends in Plant Science, 1, 215-222. [DOI:10.1016/S1360-1385(96)86898-0]
90. Prevost, A., Wilkinson, M.J. (1999). A new system of comparing PCR primers appliedto ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics, 98, 107-112. [DOI:10.1007/s001220051046]
91. Qin, A., Ding, Y., Jian, Z., Ma, F., Worth, J.R.P., Pei, S., Xu, G., Guo, Q., Shi, Z. (2021). Low genetic diversity and population differentiation in Thuja sutchuenensis Franch., an extremely endangered rediscovered conifer species in southwestern China. Global Ecology and Conservation, 25, e01430. [DOI:10.1016/j.gecco.2020.e01430]
92. Rajwade, A.V., Arora, R.S., Kadoo, N.Y., Harsulkar, A.M., Ghorpade, P.B., Gupta, V.S. (2010). Relatedness of Indian flax Genotypes (Linum usitatissimum L.): An inter-simple sequence repeat (ISSR) primer assay. Molecular Biotechnology, 45, 161-170. [DOI:10.1007/s12033-010-9256-7]
93. Reche, J., García-Martínez, S., Carbonell, P., Almansa, M.S., Hernández, F., Legua, P., Amorós, A. (2019). Relationships between physico-chemical and functional parameters and genetic analysis with ISSR markers in Spanish jujubes (Ziziphus jujuba Mill.) cultivars. Scientia Horticulturae, 253, 390-398. [DOI:10.1016/j.scienta.2019.04.068]
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Rahimian Boogar A, Salehi H. ISSR-based genetic diversity assessment of five populations of Juniperus polycarpos K. Koch in southern habitats of Iran. FOP 2021; 6 (1) :1-12
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گل و گیاهان زینتی Flower and Ornamental Plants
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