Flower and Ornamental Plants
گل و گیاهان زینتی
FOP
Agriculture
http://flowerjournal.ir
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2676-5993
2676-5993
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10.61882/flowerjournal
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fa
jalali
1401
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gregorian
2023
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بررسی نقش پوترسین، IBA و زخمزنی بر ریشهزایی قلمه ورد مینیاتور رقم ساناز زرد (Rosa hybrida L. cv. Sanaz-e-Zard) در شرایط گلخانه و کشت درون شیشهای
Investigation the effect of putrescine, IBA and wounding on rooting of cutting of Rosa hybrida cv. Sanaz-e-Zard under greenhouse and in vitro conditions
تخصصي
Special
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:115%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">ورد مینیاتور گونهای زینتی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">است که بهصورت گسترده در فضای سبز و بهعنوان گیاه گلدانی و شاخهبریدنی مورداستفاده قرار میگیرد</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:BLotus">.</span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> استفاده از روشهای سنتی برای ازدیاد این گونه­ها با محدودیت زمانی و کمبود پایه همراه است، و روشهای ریزازدیادی روبه افزایش است</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:BLotus">.</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">ریشهدار کردن قلمههای گل </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">ورد </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">بهواسطهی تنظیمکنندههای رشدی مختلف مورد اهمیت میباشد. در پژوهش حاضر نقش غلظتهای مختلف پوترسین و ایندول</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">-3-</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> بوتریک اسید (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">IBA</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">)، </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">بهصورت توأم </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">و ایجاد زخمزنی انتهای قلمه بر ریشهزایی قلمههای </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">ورد </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">میناتوری</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">رقم ساناز زرد <i>(</i></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Rosa hybrida </span></span></span></i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">cv. Sanaz-e-Zard</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">) در شرایط گلخانه و کشت درون شیشهای مورد بررسی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">قرار گرفت. </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">ریشهزایی در ریز قلمهها بر روی محیط کشت پایه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:BLotus">MS</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">حاوی پنج غلظت (0، 25/0، 5/0، 1 و 2 میلیگرم در لیتر) </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:BLotus">IBA</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">و پوترسین بررسی شد. قلمهها نیز با </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">پنج سطح (صفر، 25/0، 5/0، 1 و 2 </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:115%"></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">گرم بر لیتر) </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">IBA</span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">،</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">پوترسین و دو سطح زخمزنی (با و بدون زخم) تیمار شدند. آزمایش­ها بهصورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار صورت گرفت. نتایج در شرایط گلخانه نشان داد که بیشترین </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">طول و </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">تعداد ریشه در تیمار 1 گرم در لیتر </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">IBA</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">(بهترتیب </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">5/31 سانتیمتر و</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> 50/6 عدد) حاصل شد. زخمزنی انتهای قلمه 33/33 درصد تعداد ریشه را افزایش داد. در شرایط درون شیشه نیز بیشترین تعداد ریشه در تیمار 1 میلی‏گرم در لیتر </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">IBA</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> همراه با 2 میلی‏گرم در لیتر پوترسین (6 عدد) و بیشترین تعداد شاخساره با کاربرد همزمان 25/0، 5/0 و 2 گرم در لیتر </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">IBA</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">+ تمام سطوح پوترسین حاصل شد. </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">بهصورت کلی بهنظر میرسد که کاربرد توأم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">IBA</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">و پوترسین موجب افزایش ریشهزایی قلمههای </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">ورد </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">مینیاتور در شرایط درون و برون شیشهای میگردد. همچنین زخمزنی انتهای قلمهها نقش مؤثری در بهبود ریشهزایی در محیط گلخانه ایفا میکند.</span></span></span></span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><span new="" roman="" style="font-family:" times="">Miniature roses are hybrid roses that are widely used in landscape or as pot plants and cut flowers. Traditional methods of propagation for this species is associated with time limitation and rootstock propagule, and micropropagation techniques are increasing. Root induction of roses cuttings through different plant growth regulators is important. In this study, the effects of different concentrations of Indole-3-Butyric Acid (IBA), putrescine and wounding the end of cuttings on rooting of <i>Rosa hybrida </i>cv. SanaZard under <i>in vitro</i> and greenhouse conditions was investigated. </span><span lang="EN" new="" roman="" style="font-family:" times="">Micro-cuttings were planted in MS basal culture medium containing five concentrations (0, 0.25, 0.5, 1 and 2 mg L<sup>-1</sup>) of IBA and putrescine. In the greenhouse, t</span><span lang="EN" new="" roman="" style="font-family:" times="">he cuttings were treated with five levels of IBA, putrescine (0, 0.25, 0.5, 1 and 2 g L<sup>-1</sup>), and two levels of wounding (with and without wounding) and planted in pots after application of treatments. This study was conducted as a factorial experiment in a completely randomized design with three replicates.</span> <span lang="EN" new="" roman="" style="font-family:" times="">The results of greenhouse experiments showed that the maximum length and number of roots were obtained under the treatment of 1 </span><span lang="EN" new="" roman="" style="font-family:" times="">g L<sup>-1</sup></span><span lang="EN" new="" roman="" style="font-family:" times="">of IBA (31.5 cm and 6.50, respectively). Wounding the end of the cutting increased the number of roots by 33.33%. </span><span lang="EN" new="" roman="" style="font-family:" times="">In <i>in vitro</i> condition, the highest number of roots was observed in 1 </span><span lang="EN" new="" roman="" style="font-family:" times="">mg L<sup>-1 </sup></span><span lang="EN" new="" roman="" style="font-family:" times="">IBA + 2 </span><span lang="EN" new="" roman="" style="font-family:" times="">mg L<sup>-1</sup></span><span lang="EN" new="" roman="" style="font-family:" times="">putrescine (6) treatment </span><span lang="EN" new="" roman="" style="font-family:" times="">and the highest number of shoots were obtained with the simultaneous application of 0.25, 0.5 and 2 g L<sup>-1 </sup>of IBA + all levels of putrescine</span><span lang="EN" new="" roman="" style="font-family:" times="">. </span><span lang="EN" new="" roman="" style="font-family:" times="">In general, it seems that the combined use of IBA and putrescine could increase the rooting of miniature rose cuttings in <i>in vitro </i>and in situ conditions. Also, wounding the ends of cuttings plays an important role on inducing the roots under greenhouse environment.</span></span></span></span></div>
ایندول-3- بوتریک اسید, پرآوری, ریز قلمه, کشتبافت, محیطکشت
Culture medium, Indole-3-butyric acid, Micro-cutting, Proliferation, Tissue culture
155
170
http://flowerjournal.ir/browse.php?a_code=A-10-32-1&slc_lang=fa&sid=1
سید محمد
موسوی احمدآباد
seyedmohamad.mosavi@modares.ac.ir