Flower and Ornamental Plants
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بررسی همبستگی و واکاوی خوشهای صفات تنژگی بذر ٥ رقم ارکید فالانوپسیس در محیط کشت Chen
Study of correlation and cluster analysis of seed germination characteristics in 5 cultivars of Phalaenopsis orchid in Chen medium
تخصصي
Special
پژوهشي
Research
<div style="text-align: justify;"><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">امروزه </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">فنون کشت</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> درون</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">شیشه</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">ای برای افزایش اقتصادی و سریع گونه</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">های </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">ارکید</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> به</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">ویژه فالانوپسیس پیشنهاد می</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">شود. هدف از این پژوهش بررسی کیفیت </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تنژگی</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> بذر پنج رقم </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">ارکید</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> فالانوپسیس و همبستگی شاخصهای آن در محیط کشت</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"> Chen</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بود.</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بررسی حاضر روی بذور نارس و رسیده پنج رقم </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">ارکید</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> فالانوپسیس شامل</span></span></span><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">"</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;">Dubrovnik</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;">Memphis</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">،</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;">Andorra </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;">Bucharest</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;">Nottingham</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">انجام شد. کپسول</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">ها در هر یک از ارقام با خودگرده</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">افشانی گل تشکیل گردید. بذور در محیط کشت </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;">chen</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> کشت شدند. در فاصله 18</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تا</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">33 روز پس از کاشت، تعداد بذور نتنژیده و تنژیده شمارش گردیدند. بیشترین ضریب همبستگی بین سرعت </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تنژگی</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">و شاخص </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تنژگی</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> با مقدار 0/98 بود. نتایج </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">واکاوی</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">خوشه ای بر اساس تمامی صفات اندازهگیری شده </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تنژگی</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> به روش بیشترین میانگین فاصله بین خوشه</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">ها انجام شد که در نهایت در فاصله اقلیدوسی 1/25 ارقام به دو گروه تقسیمبندی</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">شدند</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">که</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">ارقام</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;">Dubrovnik</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;">Memphis</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">گروه اول و ارقام </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;">Andorra</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;">Bucharest</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;">Notingham</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> در گروه دوم دسته بندی شدند. صفات مهم و کلیدی در جداسازی </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">خوشهای</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> بین گروه</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">ها درصد </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تنژگی</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">، سرعت </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تنژگی</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">، شاخص </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تنژگی</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و میانگین زمان </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تنژگی</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> بودند. آزمون </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">واکاوی</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> تابع تشخیص،</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">گروه</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بندی بین ارقام را 100% تایید کرد. دو آزمون </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;">Hotelling-Lawley Trace</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;">Roy's Greatest Root</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> دارای بیشترین مقدار (5/23) برای تایید گروه</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بندی بود. با توجه به نتایج </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">واکاوی</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">خوشهای</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و همبستگی می توان نتیجه گرفت که رقم </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;">Notingham</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> دارای بیشترین مقدار در </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">عامل</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">های </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تنژگی</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> بوده و بر این اساس می</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">توان از صفات درصد </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تنژگی</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">، سرعت </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تنژگی</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و شاخص </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تنژگی</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> به عنوان صفات کلیدی برای برنامههای بهنژادی استفاده کرد.</span></span></span></div>
<div style="text-align: justify;">Nowadays, <em>in vitro</em> techniques are proposed for commercial and rapid propagation of <em>Phalaenopsis </em>species. The present study aims were investigating the quality of seed germination of 5 cultivars of <em>Phalaenopsis</em> orchid, and correlation of their traits in the culture medium. The study was conducted using the green and ripe seeds in terms of 5 cultivars of orchid as Dubrovnik, Memphis, Andorra, Bucharest and Nottingham in a factorial experiment based on a completely randomized design. The capsules were formed using self-pollination of flowers in each cultivar. The green seeds were harvested at the stage of completely formed capsules and the ripe ones were harvested at the stage of initial browning of the capsule shell. The seeds were cultured on Chen's culture medium. The number of germinated and non-germinated seeds were counted 18-33 days after their culture. The following factors were measured for each treatment: germination percentage, germination rate, germination index, mean daily germination, mean germination time and coefficient of germination rate. Correlation coefficient of %98 between the measured characteristics indicated a significant correlation between germination rate and germination index. The results of cluster analysis that was conducted based on all the measured germination characteristics showed the maximum mean distance between clusters; ultimately, that separated into two groups at Euclidean distance of 1.25 which Dubrovnik and Memphis cultivars were classified in the first group and Andorra, Bucharest and Noting<a name="_GoBack"></a>ham cultivars were classified in the second group. The key characteristics in separating cluster among the groups were considered to be germination percentage, germination rate, germination index and mean germination time. Discrimination function analysis test confirmed the grouping among the cultivars in a 100% manner. Both Hoteling-Lawley Trace as well as Roy's Greatest Root tests showed the highest value of 5.23 to confirm the grouping. It is concluded that using cluster analysis and correlation coefficient Notingham had the highest value in germination factors based on the germination percentage, germination rate and germination index which were regarded as key traits for the breeding programs.</div>
ارکید, بذر, افزایش, تنژگی
Orchid, Seed, Propagation, Germination
101
114
http://flowerjournal.ir/browse.php?a_code=A-10-44-1&slc_lang=fa&sid=1
Fatemeh
Bidarnamani
فاطمه
بیدرنامنی
f.bidarnamani65@uoz.ac.ir
10031947532846001126
10031947532846001126
Yes
Zanjan University
دانشگاه زنجان
Seyyed Najmoddin
Mortazavi
سید نجم الدین
مرتضوی
mortazavi46@gmail.com
10031947532846001127
10031947532846001127
No
university of zanjan
دانشگاه زنجان
Maryam
Rahimi
مریم
رحیمی
mrahimi@uoz.ac.ir
10031947532846001128
10031947532846001128
No
university of zabol
دانشگاه زابل