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Enhanced efficiency of acclimatization in tissue culture-derived Sansevieria trifasciata using proline, salicylic acid treatment, and endophytic fungi inoculation
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Mahboobeh Mazinani   , Mostafa K. Sarmast * , Azim Ghasemnezhad , Liang Cheng |
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Abstract: (57 Views) |
| Tissue culture and initial acclimatization of many ornamental species have become feasible; however, the growth and development of tissue-cultured plants after transfer to soil substrates in the greenhouse typically proceed slowly. This research investigates the effects of proline, salicylic acid, and endophytic fungi on enhancing the acclimatization of Sansevieria plantlets following their transfer to the soil environment. This study investigated the effects of proline, salicylic acid, and endophytic fungi on improving the acclimation of Sansevieria plantlets. Rooted plantlets were treated with proline and salicylic acid solutions (each at 10 and 100 µM), as well as with spore suspensions of the endophytic fungi Cladosporium limoniforme and Penicillium biourgeianum (for 1 and 3 hours). Evaluation five months post-treatment revealed that the application of endophytic fungi, especially the 3-hour treatment, was the most effective. These treatments significantly increased leaf length (up to 25%), root number (up to 35%), and the content of total chlorophyll and carotenoids compared to the control. In contrast, although proline (100 µM) increased leaf proline accumulation by 40%, it did not lead to significant improvement in growth indices. The results confirm the superiority of the biological approach using endophytes in facilitating acclimation and enhancing vegetative growth of Sansevieria after transfer to the greenhouse. To develop this method, future studies could focus on the molecular mechanisms of this interaction and the combined application of these stimulants with other agents. |
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| Keywords: acclimatization, Endophyte, root, micropropagation |
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Type of Study: Research |
Subject:
Special
Received: 2026/02/14 | Accepted: 2026/09/22 | Published: 2026/10/27
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