Breeding and production of new varieties of ornamental plants as well as their propagation and development is an expanding industry. The application of ornamental plants in landscape and increasing the demand for new varieties has led the breeders to plan for production of new varieties with attractive colors, excessive perfumes, more durability and resistance to biotic and abiotic stresses. Generally, diversity is a basic principle in any breeding program and plant breeding relies on variety, selection, evaluation and production of new genotypes. One way to increase diversity is mutation induction at in vitro conditions. Application of this method can help to increase diversity and enhance the efficiency of breeding programs and improve or achieve useful traits which have been lost in the plants evolution process. This article reviews the new research outcomes for increasing the diversity via induced mutation of ornamental plants combined with modern methods such as plant tissue culture and molecular markers. According to this study, the application of mutagens at in vitro conditions, using the molecular markers for selection of mutant plants in the early stages and increasing the rate of their propagation for enhancing the efficiency of breeding of ornamental plants is suggested.