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:: دوره 7، شماره 1 - ( بهار و تابستان 1401 ) ::
جلد 7 شماره 1 صفحات 26-13 برگشت به فهرست نسخه ها
بررسی رابطه بین گونه‎‌های پلاخور (Lonicera spp.) موجود در ایران با استفاده از رمزینه کردن DNA و شاخص‌‌های ریخت‌شناسانه
نجمه فتاحی دهکردی ، مسعود قاسمی قهساره* ، بهروز شیران
دانشگاه شهرکرد
چکیده:   (1426 مشاهده)
جنس Lonicera از تیره Caprifoliaceae است و در زبان فارسی به نام‌های پلاخور، شونگ و یا پیچ امین‌الدوله (Honeysuckle) خوانده می‌شود. شناخت روابط بین گونه‌ای بر اساس ویژگی‌های ریخت‌‌شناسانه و بیوشیمیایی گاهی نتایج متناقضی داشته‌اند. در این مطالعه، برای شناسایی، جداسازی و تعیین روابط تبارزایی 12 گونه Lonicera موجود درایران، از نشانگرهای ریخت شناسانه، رمزینه‌های DNA هسته‌ای و کلروپلاستی (شامل ITS و matK) همراه با چند توالی دیگر از پایگاه بانک ژن NCBI استفاده گردید. DNA ژنومی با استفاده از کیت‌‌های موجود استخراج و واکنش PCR برای تکثیر دو ناحیه مورد نظر انجام شد و در پایان نمونه‌‌های خالص‌‌سازی شده توالی یابی شدند. تجزیه خوشه‌‌ای بر اساس ویژگی‌های ریخت‌‌شناسانه‌‌، گونه‌‌ها را در دو گروه بزرگ قرار داد که گروه اول شامل L. sempervirens و گروه دوم شامل 11 گونه‌‌ دیگر بود. نزدیکترین رابطه ژنتیکی بین گونه‌‌های L. floribunda و L. nummulariifolia و کمترین شباهت بین گونه‌‌های L. sempervirens و L. caprifolium وجود داشت. واکاوی به عامل‌‌های اصلی (Principle component analysis (PCAs)) روی داده‌‌های مربوط به ریخت‌‌شناسی نشان داد که سه عامل اول در مجموع1/71 % گوناگونی را شامل می‌‌شدند که عامل اول شامل پوشش کرکی ساقه، شکل پهنک، حاشیه برگ، شکل نوک پهنک، رنگ گل، پوشش کرکی برگ، پایین برگ و رنگ میوه، طول دمبرگ و فاصله میانگره به عنوان اجزاء اصلی، 8/38% گوناگونی را توجیه نمود. بر اساس بررسی‌‌های مولکولی، طول منطقه matK در حدود 1135-1110 نوکلئوتید بود. آنالیز پارسیمونی این ناحیه 944 جایگاه حفاظت شده، 109 جایگاه متغیر و 19 جایگاه پارسیمون را نشان داد. در درخت تبارزایی ایجاد شده بوسیله‌ی ناحیه matK جداسازی همه‌‌ی گونه‌‌ها به جز دو گونه L. korolkovii و L. maackii به خوبی صورت گرفته و در شاخه‌های جداگانه قرار گرفتند. گوناگونی و فاصله ژنتیکی بین گونه‌‌ها در ناحیه matK با فاصله ژنتیکی 00/0 تا 057/0 مشاهده شد. این مطالعه نتوانست از آغازگرهای ITS جواب مطلوبی حاصل نماید. بنابراین، ناحیه matK با توجه به گوناگونی و تکثیر آسان، رمزینه مناسبی برای بررسی گوناگونی ژنتیکی بین گونه‌‌های پلاخور است.

 
واژه‌های کلیدی: نشانگرهای ریخت‌‌شناسانه، نواحی بارکد، فیلوژنی، امین‌الدوله، matK
متن کامل [PDF 1021 kb]   (180 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: تخصصي
دریافت: 1400/12/3 | پذیرش: 1401/1/18 | انتشار: 1401/7/8
فهرست منابع
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2. Arzanlo, M., Dokhanchi, H., Davari, M., Khodaveisi, S., Badali, H. (2013). DNA Barcoding: New Horizons in Molecular Identification of Pathogenic Fungi (Review Article). Journal of Kurdistan University of Medical Sciences, 18, 113-126 (In Persian).
3. Castro, C., Hernandez, A., Alvarado, L., Flores, D. (2015). DNA barcodes in fig cultivars (Ficus carcia) uses ITS regions of ribosomal DNA, the trnH-psbA spacer and the matK coding sequence. American Jonrual of Plant Sciences, 6(01), 95-102. [DOI:10.4236/ajps.2015.61011]
4. Chen, W.C., Liou, S.S., Tzeng, T.F., Lee, S.L., Liu, I.M. (2012). Wound repair and anti-inflammatory potential of Lonicera japonica in excision wound induced rats. BMC Complementary and Alternative Medicine, 12(1), 1-9. [DOI:10.1186/1472-6882-12-226]
5. Desalle, R.O.B. (2006). Species discovery versus species identification in DNA barcoding efforts: response to Rubinoff. Conservation Biology, 20(5), 1545- 547. [DOI:10.1111/j.1523-1739.2006.00543.x]
6. Drabkova, L., Kirschner, J., Vlček, Č., Pačes, V. (2004). TrnL-trnF intergenic spacer and trnL intron define major clades within Luzula and Juncus (Juncaceae): importance of structural mutations. Journal of Molecular Evolution, 59(1), 1-10. [DOI:10.1007/s00239-004-2598-7]
7. Dunning, L.T., Savolainen, V. (2010). Broad-scale amplification of matK for DNA barcoding. Botanical Journal of the Linnean Society, 164(1), 1-9. [DOI:10.1111/j.1095-8339.2010.01071.x]
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9. Feng, S., Jing, Y., Wang, S., Jiang, M., Chen, Z., Ying, Q., Wang, H. (2015). Molecular identification of Dendrobium species (Orchidaceae) based on the DNA barcode ITS2 region and its application for phylogenetic study. International Journal of Molecular Sciences, 16(9), 21975-21988. [DOI:10.3390/ijms160921975]
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12. Hebert, P.D., Ratnasingham, S., De Waard, J.R. (2003). Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Biological Sciences, 270, 96-99. [DOI:10.1098/rsbl.2003.0025]
13. Hong-Jin, D.O.N.G., Hua, P.E.N.G. (2014). Relationships within the Lonicera macrantha complex based on morphological and molecular data. Plant Diversity and Resources, 36(2), 133-142.
14. Ipek, M., Ipek, A., Simon, P.W. (2014). Testing the utility of matK and ITS DNA regions for discrimination of Allium species. Turkish Journal Botanical, 38(2), 203-212. [DOI:10.3906/bot-1308-46]
15. Jalili, S., Hamdi, S.M.M., Oraghiardebili, Z. (2017) Anatomical characteristics of the leaves and stems of 9 species of the genus Lonicera from Caprifoliaceae family in Iran. Journal of Plant Research (Iranian Journal of Biology), 29(4), 751-765. (In Persian).
16. Johnson, L. A., Chan, L. M. Weese, Weese, L. D., Mcmurry, S. (2008). Nuclear and cpDNA sequences combined provide strong inference of higher phylogenetic relationships in the phlox family (Polemoniaceae). Molecular Phylogenetics and Evolution, 48(3), 997-1012. [DOI:10.1016/j.ympev.2008.05.036]
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23. Qing Ren, B., Guo xiang, X., Duan chen, Z. (2010). Species identification of Alnus (Betulaceae) using nrDNA and cpDNA genetic markers. Molecular Ecology Resources, 10(4), 594-605. [DOI:10.1111/j.1755-0998.2009.02815.x]
24. Schöttler, M.A., Flügel, C., Thiele, W., Stegemann, S., Bock, R. (2007). The plastome-encoded PsaJ subunit is required for efficient photosystem I excitation, but not for plastocyanin oxidation in tobacco. Biochemical Journal, 403(2), 251-260. [DOI:10.1042/BJ20061573]
25. Sun, Z., Gao, T. Yao, H., Shi, L., Zhu. Y., Chen, S. (2011). Identification of Lonicera japonica and its related species using the DNA barcoding method. Planta Medica, 77(03), 301-306 [DOI:10.1055/s-0030-1250324]
26. Taberlet, P., Coissac, E., Pompanon, F., Gielly, L. Miquel, C. (2007). Power and limitations of the chloroplast trnL (UAA) intron for plant DNA barcoding. Nucleic Acids Research, 35(3), 3-14. [DOI:10.1093/nar/gkl938]
27. Tamura, K., Dudley, J., Nei, M., Kumar, S. (2007). MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24(8), 1596-1599. [DOI:10.1093/molbev/msm092]
28. Theis, N., Donoghue, M.J., Li, J. (2008). Phylogenetics of the Caprifolieae and Lonicera (Dipsacales) based on nuclear and chloroplast DNA sequences. Systematic Botany, 33(4), 776-783. [DOI:10.1600/036364408786500163]
29. Wang, M., Zhao, H.X., Wang, L., Wang, T., Yang, R.W., Wang, X.L., Zhou, Y.H., Ding, C.B., Zhang, L. (2013). Potential use of DNA barcoding for the identification of Salvia based on cpDNA and nrDNA sequences. Gene, 528(2), 206-215. [DOI:10.1016/j.gene.2013.07.009]
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31. Yao, P.C., Gao, H.Y., Wei, Y.N., Zhang, J.H. Chen, X.Y., Li, H.Q. (2017). Evaluating sampling strategy for DNA barcoding study of coastal and inland halo-tolerant Poaceae and Chenopodiaceae: a case study for increased sample size. Plos One, 12(9), 0185311. [DOI:10.1371/journal.pone.0185311]
32. Alian, M. (2013). Phylogenetic analysis of Stachys L. species in Central Zagros region of Iran using sequencing of ribosomal ITS DNA ITS trnL-trnF chloroplasts. PhD Thesis, University of Shahrekord, Iran. (In Persian).
33. Arzanlo, M., Dokhanchi, H., Davari, M., Khodaveisi, S., Badali, H. (2013). DNA Barcoding: New Horizons in Molecular Identification of Pathogenic Fungi (Review Article). Journal of Kurdistan University of Medical Sciences, 18, 113-126 (In Persian).
34. Castro, C., Hernandez, A., Alvarado, L., Flores, D. (2015). DNA barcodes in fig cultivars (Ficus carcia) uses ITS regions of ribosomal DNA, the trnH-psbA spacer and the matK coding sequence. American Jonrual of Plant Sciences, 6(01), 95-102. [DOI:10.4236/ajps.2015.61011]
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36. Desalle, R.O.B. (2006). Species discovery versus species identification in DNA barcoding efforts: response to Rubinoff. Conservation Biology, 20(5), 1545- 547. [DOI:10.1111/j.1523-1739.2006.00543.x]
37. Drabkova, L., Kirschner, J., Vlček, Č., Pačes, V. (2004). TrnL-trnF intergenic spacer and trnL intron define major clades within Luzula and Juncus (Juncaceae): importance of structural mutations. Journal of Molecular Evolution, 59(1), 1-10. [DOI:10.1007/s00239-004-2598-7]
38. Dunning, L.T., Savolainen, V. (2010). Broad-scale amplification of matK for DNA barcoding. Botanical Journal of the Linnean Society, 164(1), 1-9. [DOI:10.1111/j.1095-8339.2010.01071.x]
39. Fatahi, N., Ghasemi ghehsare, M., Shiran, B., Siampoor, M. (2021). The investigation of chloroplast marker psbA-trnH and trnL-F in determining the relationships between species of Lonicera spp. In: Third International and Fourth National Congress of Flowers and Ornamental Plants of Iran. (In Persian).
40. Feng, S., Jing, Y., Wang, S., Jiang, M., Chen, Z., Ying, Q., Wang, H. (2015). Molecular identification of Dendrobium species (Orchidaceae) based on the DNA barcode ITS2 region and its application for phylogenetic study. International Journal of Molecular Sciences, 16(9), 21975-21988. [DOI:10.3390/ijms160921975]
41. Ghasemi Ghehsareh, M., Kafi, M. (2015). Scientific and Practical Floriculture. Moallef Press. 296p. (In Persian).
42. Hajibabaei, M., Singer, G.A., Hebert, P.D., Hickey, D.A. (2007). DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics. Trend in Genetics, 23(4), 167-172. [DOI:10.1016/j.tig.2007.02.001]
43. Hebert, P.D., Ratnasingham, S., De Waard, J.R. (2003). Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Biological Sciences, 270, 96-99. [DOI:10.1098/rsbl.2003.0025]
44. Hong-Jin, D.O.N.G., Hua, P.E.N.G. (2014). Relationships within the Lonicera macrantha complex based on morphological and molecular data. Plant Diversity and Resources, 36(2), 133-142.
45. Ipek, M., Ipek, A., Simon, P.W. (2014). Testing the utility of matK and ITS DNA regions for discrimination of Allium species. Turkish Journal Botanical, 38(2), 203-212. [DOI:10.3906/bot-1308-46]
46. Jalili, S., Hamdi, S.M.M., Oraghiardebili, Z. (2017) Anatomical characteristics of the leaves and stems of 9 species of the genus Lonicera from Caprifoliaceae family in Iran. Journal of Plant Research (Iranian Journal of Biology), 29(4), 751-765. (In Persian).
47. Johnson, L. A., Chan, L. M. Weese, Weese, L. D., Mcmurry, S. (2008). Nuclear and cpDNA sequences combined provide strong inference of higher phylogenetic relationships in the phlox family (Polemoniaceae). Molecular Phylogenetics and Evolution, 48(3), 997-1012. [DOI:10.1016/j.ympev.2008.05.036]
48. Hartmann, H. T., Kester, D. E. (2011). Hartmann and Kester's Plant Propagation: Principles and Practices. Pearson Prentice Hall. 922p.
49. Khatamsaz, M. (1995). Flora of Iran, No. 13, Caprifiliaceae. Research Instutute og Forest and Rangelands. 32p. (In Persian).
50. Kovach, W. L. (1999). MVSP-A multivariate statistical Package for Windows, ver. 3.1. Kovach Computing Services, Pentraeth, Wales, UK. 137p.
51. Lahaye, R., Van der Bank, M., Bogarin, D., Warner, J., Pupulin, F., Gigot, G., Maurin, O., Duthoit, S., Barraclough, T.G., Savolainen, V. (2008). DNA barcoding the floras of biodiversity hotspots. Proceedings of the National Academy of Sciences, 105(8), 2923-2928. [DOI:10.1073/pnas.0709936105]
52. Mabberley, D.J. (2008). Mabberley's Plant-book: A Portable Dictionary of Plants, their Classification and Uses. Cambridge University Press. 1040P.
53. Pang, X., Song, J. Zhu, Y., Xie, C., Chen, S. (2010). Using DNA barcoding to identify species within euphorbiaceae. Planta Medica, 76(15), 1784-1786. [DOI:10.1055/s-0030-1249806]
54. Qing Ren, B., Guo xiang, X., Duan chen, Z. (2010). Species identification of Alnus (Betulaceae) using nrDNA and cpDNA genetic markers. Molecular Ecology Resources, 10(4), 594-605. [DOI:10.1111/j.1755-0998.2009.02815.x]
55. Schöttler, M.A., Flügel, C., Thiele, W., Stegemann, S., Bock, R. (2007). The plastome-encoded PsaJ subunit is required for efficient photosystem I excitation, but not for plastocyanin oxidation in tobacco. Biochemical Journal, 403(2), 251-260. [DOI:10.1042/BJ20061573]
56. Sun, Z., Gao, T. Yao, H., Shi, L., Zhu. Y., Chen, S. (2011). Identification of Lonicera japonica and its related species using the DNA barcoding method. Planta Medica, 77(03), 301-306 [DOI:10.1055/s-0030-1250324]
57. Taberlet, P., Coissac, E., Pompanon, F., Gielly, L. Miquel, C. (2007). Power and limitations of the chloroplast trnL (UAA) intron for plant DNA barcoding. Nucleic Acids Research, 35(3), 3-14. [DOI:10.1093/nar/gkl938]
58. Tamura, K., Dudley, J., Nei, M., Kumar, S. (2007). MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24(8), 1596-1599. [DOI:10.1093/molbev/msm092]
59. Theis, N., Donoghue, M.J., Li, J. (2008). Phylogenetics of the Caprifolieae and Lonicera (Dipsacales) based on nuclear and chloroplast DNA sequences. Systematic Botany, 33(4), 776-783. [DOI:10.1600/036364408786500163]
60. Wang, M., Zhao, H.X., Wang, L., Wang, T., Yang, R.W., Wang, X.L., Zhou, Y.H., Ding, C.B., Zhang, L. (2013). Potential use of DNA barcoding for the identification of Salvia based on cpDNA and nrDNA sequences. Gene, 528(2), 206-215. [DOI:10.1016/j.gene.2013.07.009]
61. White, T.J., Bruns, T., Lee, S., Taylor, J. (1990). Amplification and Direct Sequencing of Fungal Ribosomal RNA Gens for Phylogenetics. In: Innis M.A. et al. (eds.), PCR Protocols: A Guide to Methods and Applications. Academic Press. New York, pp. 315-322 [DOI:10.1016/B978-0-12-372180-8.50042-1]
62. Yao, P.C., Gao, H.Y., Wei, Y.N., Zhang, J.H. Chen, X.Y., Li, H.Q. (2017). Evaluating sampling strategy for DNA barcoding study of coastal and inland halo-tolerant Poaceae and Chenopodiaceae: a case study for increased sample size. Plos One, 12(9), 0185311. [DOI:10.1371/journal.pone.0185311]
63. Alian, M. (2013). Phylogenetic analysis of Stachys L. species in Central Zagros region of Iran using sequencing of ribosomal ITS DNA ITS trnL-trnF chloroplasts. PhD Thesis, University of Shahrekord, Iran. (In Persian).
64. Arzanlo, M., Dokhanchi, H., Davari, M., Khodaveisi, S., Badali, H. (2013). DNA Barcoding: New Horizons in Molecular Identification of Pathogenic Fungi (Review Article). Journal of Kurdistan University of Medical Sciences, 18, 113-126 (In Persian).
65. Castro, C., Hernandez, A., Alvarado, L., Flores, D. (2015). DNA barcodes in fig cultivars (Ficus carcia) uses ITS regions of ribosomal DNA, the trnH-psbA spacer and the matK coding sequence. American Jonrual of Plant Sciences, 6(01), 95-102. [DOI:10.4236/ajps.2015.61011]
66. Chen, W.C., Liou, S.S., Tzeng, T.F., Lee, S.L., Liu, I.M. (2012). Wound repair and anti-inflammatory potential of Lonicera japonica in excision wound induced rats. BMC Complementary and Alternative Medicine, 12(1), 1-9. [DOI:10.1186/1472-6882-12-226]
67. Desalle, R.O.B. (2006). Species discovery versus species identification in DNA barcoding efforts: response to Rubinoff. Conservation Biology, 20(5), 1545- 547. [DOI:10.1111/j.1523-1739.2006.00543.x]
68. Drabkova, L., Kirschner, J., Vlček, Č., Pačes, V. (2004). TrnL-trnF intergenic spacer and trnL intron define major clades within Luzula and Juncus (Juncaceae): importance of structural mutations. Journal of Molecular Evolution, 59(1), 1-10. [DOI:10.1007/s00239-004-2598-7]
69. Dunning, L.T., Savolainen, V. (2010). Broad-scale amplification of matK for DNA barcoding. Botanical Journal of the Linnean Society, 164(1), 1-9. [DOI:10.1111/j.1095-8339.2010.01071.x]
70. Fatahi, N., Ghasemi ghehsare, M., Shiran, B., Siampoor, M. (2021). The investigation of chloroplast marker psbA-trnH and trnL-F in determining the relationships between species of Lonicera spp. In: Third International and Fourth National Congress of Flowers and Ornamental Plants of Iran. (In Persian).
71. Feng, S., Jing, Y., Wang, S., Jiang, M., Chen, Z., Ying, Q., Wang, H. (2015). Molecular identification of Dendrobium species (Orchidaceae) based on the DNA barcode ITS2 region and its application for phylogenetic study. International Journal of Molecular Sciences, 16(9), 21975-21988. [DOI:10.3390/ijms160921975]
72. Ghasemi Ghehsareh, M., Kafi, M. (2015). Scientific and Practical Floriculture. Moallef Press. 296p. (In Persian).
73. Hajibabaei, M., Singer, G.A., Hebert, P.D., Hickey, D.A. (2007). DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics. Trend in Genetics, 23(4), 167-172. [DOI:10.1016/j.tig.2007.02.001]
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105. Hebert, P.D., Ratnasingham, S., De Waard, J.R. (2003). Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Biological Sciences, 270, 96-99. [DOI:10.1098/rsbl.2003.0025]
106. Hong-Jin, D.O.N.G., Hua, P.E.N.G. (2014). Relationships within the Lonicera macrantha complex based on morphological and molecular data. Plant Diversity and Resources, 36(2), 133-142.
107. Ipek, M., Ipek, A., Simon, P.W. (2014). Testing the utility of matK and ITS DNA regions for discrimination of Allium species. Turkish Journal Botanical, 38(2), 203-212. [DOI:10.3906/bot-1308-46]
108. Jalili, S., Hamdi, S.M.M., Oraghiardebili, Z. (2017) Anatomical characteristics of the leaves and stems of 9 species of the genus Lonicera from Caprifoliaceae family in Iran. Journal of Plant Research (Iranian Journal of Biology), 29(4), 751-765. (In Persian).
109. Johnson, L. A., Chan, L. M. Weese, Weese, L. D., Mcmurry, S. (2008). Nuclear and cpDNA sequences combined provide strong inference of higher phylogenetic relationships in the phlox family (Polemoniaceae). Molecular Phylogenetics and Evolution, 48(3), 997-1012. [DOI:10.1016/j.ympev.2008.05.036]
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113. Lahaye, R., Van der Bank, M., Bogarin, D., Warner, J., Pupulin, F., Gigot, G., Maurin, O., Duthoit, S., Barraclough, T.G., Savolainen, V. (2008). DNA barcoding the floras of biodiversity hotspots. Proceedings of the National Academy of Sciences, 105(8), 2923-2928. [DOI:10.1073/pnas.0709936105]
114. Mabberley, D.J. (2008). Mabberley's Plant-book: A Portable Dictionary of Plants, their Classification and Uses. Cambridge University Press. 1040P.
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116. Qing Ren, B., Guo xiang, X., Duan chen, Z. (2010). Species identification of Alnus (Betulaceae) using nrDNA and cpDNA genetic markers. Molecular Ecology Resources, 10(4), 594-605. [DOI:10.1111/j.1755-0998.2009.02815.x]
117. Schöttler, M.A., Flügel, C., Thiele, W., Stegemann, S., Bock, R. (2007). The plastome-encoded PsaJ subunit is required for efficient photosystem I excitation, but not for plastocyanin oxidation in tobacco. Biochemical Journal, 403(2), 251-260. [DOI:10.1042/BJ20061573]
118. Sun, Z., Gao, T. Yao, H., Shi, L., Zhu. Y., Chen, S. (2011). Identification of Lonicera japonica and its related species using the DNA barcoding method. Planta Medica, 77(03), 301-306 [DOI:10.1055/s-0030-1250324]
119. Taberlet, P., Coissac, E., Pompanon, F., Gielly, L. Miquel, C. (2007). Power and limitations of the chloroplast trnL (UAA) intron for plant DNA barcoding. Nucleic Acids Research, 35(3), 3-14. [DOI:10.1093/nar/gkl938]
120. Tamura, K., Dudley, J., Nei, M., Kumar, S. (2007). MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24(8), 1596-1599. [DOI:10.1093/molbev/msm092]
121. Theis, N., Donoghue, M.J., Li, J. (2008). Phylogenetics of the Caprifolieae and Lonicera (Dipsacales) based on nuclear and chloroplast DNA sequences. Systematic Botany, 33(4), 776-783. [DOI:10.1600/036364408786500163]
122. Wang, M., Zhao, H.X., Wang, L., Wang, T., Yang, R.W., Wang, X.L., Zhou, Y.H., Ding, C.B., Zhang, L. (2013). Potential use of DNA barcoding for the identification of Salvia based on cpDNA and nrDNA sequences. Gene, 528(2), 206-215. [DOI:10.1016/j.gene.2013.07.009]
123. White, T.J., Bruns, T., Lee, S., Taylor, J. (1990). Amplification and Direct Sequencing of Fungal Ribosomal RNA Gens for Phylogenetics. In: Innis M.A. et al. (eds.), PCR Protocols: A Guide to Methods and Applications. Academic Press. New York, pp. 315-322 [DOI:10.1016/B978-0-12-372180-8.50042-1]
124. Yao, P.C., Gao, H.Y., Wei, Y.N., Zhang, J.H. Chen, X.Y., Li, H.Q. (2017). Evaluating sampling strategy for DNA barcoding study of coastal and inland halo-tolerant Poaceae and Chenopodiaceae: a case study for increased sample size. Plos One, 12(9), 0185311. [DOI:10.1371/journal.pone.0185311]
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Fattahi Dehkordi N, Ghaemi Ghehsareh M, Shiran B. Investigation of the relationship between Honeysuckle species (Lonicera spp.) in Iran using DNA barcoding and morphological markers. FOP 2022; 7 (1) :13-26
URL: http://flowerjournal.ir/article-1-222-fa.html

فتاحی دهکردی نجمه، قاسمی قهساره مسعود، شیران بهروز. بررسی رابطه بین گونه‎‌های پلاخور (Lonicera spp.) موجود در ایران با استفاده از رمزینه کردن DNA و شاخص‌‌های ریخت‌شناسانه. گل و گیاهان زینتی. 1401; 7 (1) :13-26

URL: http://flowerjournal.ir/article-1-222-fa.html



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گل و گیاهان زینتی Flower and Ornamental Plants
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